Determination of proliferation index in neoplasms using different Ki-67 equivalent antibodies
| Autor: | Sverre H. Torp, C. F. Lindboe, G. Von Der Ohe |
|---|---|
| Rok vydání: | 2003 |
| Předmět: |
Microbiology (medical)
Pathology medicine.medical_specialty biology Proliferation index Antibodies Monoclonal General Medicine Immunohistochemistry Pathology and Forensic Medicine Ki-67 Antigen Polyclonal antibodies Antibodies Antinuclear Neoplasms Ki-67 Labelling Monoclonal Mitotic Index biology.protein medicine Tumour grading Humans Immunology and Allergy Antibody |
| Zdroj: | APMIS. 111:567-570 |
| ISSN: | 1600-0463 0903-4641 |
| DOI: | 10.1034/j.1600-0463.2003.1110505.x |
| Popis: | Paraffin sections from 23 tumours were immunohistochemically stained with the following four Ki-67 equivalent antibodies: monoclonal MIB-1 (DAKO), monoclonal MM1 (Novocastra), polyclonal NCL-Ki-67p (Novocastra), and polyclonal Rah Ki-67 (DAKO). Ki-67 labelling indices were determined by counting in exactly the same area in each case. MIB-1 showed the highest labelling index in 21 of the 23 cases, and the mean MIB-1 index was approximately 30% higher than that of the other antibodies. The differences between MM1, NCL-Ki-67p and Rah Ki-67 were small and non-significant. There was a positive correlation between each of the four antibodies. As these findings may be of importance when the Ki-67 labelling index is used as a criterion for tumour grading or for clinical prognostication, this necessitate identification of the antibody used in every case. |
| Databáze: | OpenAIRE |
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