Transcription Factor RFX2 Is a Key Regulator of Mouse Spermiogenesis
| Autor: | Xiwen Lin, Zhuqiang Zhang, Yan-Ling Wang, Xiuhong Cui, Fei Gao, Zhen Li, Yujian Wu, Si Wang, Xue Feng, Xiangjing Hu, Xiuxia Wang, Chunsheng Han, Sisi Li, Rex A. Hess, Shangying Liao, Min Wang |
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| Rok vydání: | 2016 |
| Předmět: |
Male
0301 basic medicine Chromatin Immunoprecipitation Spermiogenesis Blotting Western Fluorescent Antibody Technique Apoptosis Regulatory Factor X Transcription Factors Biology Article Immunoenzyme Techniques Mice 03 medical and health sciences Spermatocytes Ciliogenesis Testis medicine Animals Spermatogenesis Transcription factor Mice Knockout Multidisciplinary Spermatid Molecular biology Chromatin 030104 developmental biology medicine.anatomical_structure Gene Expression Regulation Knockout mouse Chromatin immunoprecipitation |
| Zdroj: | Scientific Reports |
| ISSN: | 2045-2322 |
| Popis: | The regulatory factor X (RFX) family of transcription factors is crucial for ciliogenesis throughout evolution. In mice, Rfx1-4 are highly expressed in the testis where flagellated sperm are produced, but the functions of these factors in spermatogenesis remain unknown. Here, we report the production and characterization of the Rfx2 knockout mice. The male knockout mice were sterile due to the arrest of spermatogenesis at an early round spermatid step. The Rfx2-null round spermatids detached from the seminiferous tubules, forming large multinucleated giant cells that underwent apoptosis. In the mutants, formation of the flagellum was inhibited at its earliest stage. RNA-seq analysis identified a large number of cilia-related genes and testis-specific genes that were regulated by RFX2. Many of these genes were direct targets of RFX2, as revealed by chromatin immunoprecipitation-PCR assays. These findings indicate that RFX2 is a key regulator of the post-meiotic development of mouse spermatogenic cells. |
| Databáze: | OpenAIRE |
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