Overexpression of B7-H3 in CD133+ colorectal cancer cells is associated with cancer progression and survival in human patients
| Autor: | Lv Desheng, Zhang Guangbo, Ge Yan, Zhou Huan, Zhang Xue-guang, Zhou Bin |
|---|---|
| Rok vydání: | 2014 |
| Předmět: |
Adult
Male Oncology medicine.medical_specialty B7 Antigens Colon Colorectal cancer Adenocarcinoma Biology Antigens CD Internal medicine medicine Humans Clinical significance AC133 Antigen neoplasms Aged Glycoproteins Aged 80 and over Tissue microarray Tumor Immune Escape Rectum Cancer Middle Aged medicine.disease Staining carbohydrates (lipids) Biological significance Lymphatic Metastasis embryonic structures Disease Progression Immunohistochemistry Female Surgery Colorectal Neoplasms Peptides |
| Zdroj: | Journal of Surgical Research. 188:396-403 |
| ISSN: | 0022-4804 |
| DOI: | 10.1016/j.jss.2014.01.014 |
| Popis: | Cancer stem-like cells are enriched in CD133-positive (CD133(+)) colorectal cancer (CRC) cells. To date, the biological significance of CD133 expression in cancer stem-like cells is still unknown. B7-H3, a costimulatory molecule, plays a pivotal role in tumor immune escape by inhibiting the functions of T cells. To identify a new marker to predict the tumor grade of CRC, we analyzed the expression of B7-H3 and CD133 in colorectal tumor samples, and their clinical significance was determined. By using a series of techniques including pathologic tissue microarray technology, immunohistochemistry, and immunofluorescent staining, we found B7-H3 was expressed in 56.73% of the CRC cases (59/104) sampled; CD133 was detected in 26.92% of the CRC cases (28/104) sampled. Further analysis indicated that 22 of these CD133(+) samples expressed B7-H3. We also found coexpression of CD133 and B7-H3 in tumor tissue samples (r = 0.321, P 0.01). Moreover, in contrast to individual CD133 or B7-H3 expression, the coexpression of B7-H3 and CD133 was evidently associated with the depth of tumor invasion, lymphatic metastasis, distant metastasis, and Dukes' stage, suggesting it is a valuable biomarker for the progression of CRC. Indeed, the patients with coexpression of B7-H3 and CD133 had a poorer survival than the other patients (P 0.05). In summary, our results reveal that B7-H3 was aberrantly expressed in CD133(+) CRC cells, and the expression level was closely associated with tumor progression. |
| Databáze: | OpenAIRE |
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