Ready-to-Use Germanium Surfaces for the Development of FTIR-Based Biosensors for Proteins
| Autor: | Julie Hubert, Pascale Blond, Robin Bevernaegie, Ludovic Troian-Gautier, Vincent Raussens, François Reniers, Corinne Lagrost, Ivan Jabin |
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| Přispěvatelé: | Laboratoire de Chimie Organique, Université Libre de Bruxelles (LCO), Université libre de Bruxelles (ULB), Institut des Sciences Chimiques de Rennes (ISCR), Université de Rennes (UR)-Institut National des Sciences Appliquées - Rennes (INSA Rennes), Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Ecole Nationale Supérieure de Chimie de Rennes (ENSCR)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Université Libre de Bruxelles, Actions de Recherches Concerte?es? of the Fe?de?ration Wallonie-Bruxelles, Bridge ? Green Chemistry Program of Innoviris, Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC)-Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-Ecole Nationale Supérieure de Chimie de Rennes (ENSCR)-Institut National des Sciences Appliquées - Rennes (INSA Rennes), Institut National des Sciences Appliquées (INSA)-Université de Rennes (UNIV-RENNES)-Institut National des Sciences Appliquées (INSA) |
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Streptavidin
Physique de l'état condense [struct. électronique etc.] chemistry.chemical_element Germanium 02 engineering and technology Physique de l'état condense [struct. propr. thermiques etc.] 010402 general chemistry 01 natural sciences chemistry.chemical_compound Calixarene Electrochemistry [CHIM]Chemical Sciences General Materials Science Physique des surfaces Spectroscopie [état condense] Bovine serum albumin Physique de l'état condense [supraconducteur] Spectroscopy ComputingMilieux_MISCELLANEOUS chemistry.chemical_classification Bioconjugation biology Biomolecule Electrochimie hautes et basses températures technology industry and agriculture Surfaces and Interfaces 021001 nanoscience & nanotechnology Condensed Matter Physics Combinatorial chemistry 0104 chemical sciences Spectroscopie [électromagnétisme optique acoustique] chemistry Métallurgie et mines biology.protein 0210 nano-technology Biosensor Ethylene glycol |
| Zdroj: | Langmuir Langmuir, American Chemical Society, 2020, 36 (40), pp.12068-12076. ⟨10.1021/acs.langmuir.0c02681⟩ Langmuir, 2020, 36 (40), pp.12068-12076. ⟨10.1021/acs.langmuir.0c02681⟩ |
| ISSN: | 0743-7463 1520-5827 |
| Popis: | Germanium is particularly suitable for the design of FTIR-based biosensors for proteins. The grafting of stable and thin organic layers on germanium surfaces remains, however, challenging. To tackle this problem, we developed a calix[4]arene-tetradiazonium salt decorated with four oligo(ethylene glycol) chains and a terminal reactive carboxyl group. This versatile molecular platform was covalently grafted on germanium surfaces to yield robust ready-to-use surfaces for biosensing applications. The grafted calixarene monolayer prevents nonspecific adsorption of proteins while allowing bioconjugation with biomolecules such as bovine serum albumin (BSA) or biotin. It is shown that the native form of the investigated proteins was maintained upon immobilization. As a proof of concept, the resulting calix[4]arene-based germanium biosensors were used through a combination of ATR-FTIR spectroscopy and fluorescence microscopy for the selective detection of streptavidin from a complex medium. This study opens real possibilities for the development of sensitive and selective FTIR-based biosensors devoted to the detection of proteins. SCOPUS: ar.j info:eu-repo/semantics/published |
| Databáze: | OpenAIRE |
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