Germanium nanoparticles: Intrinsic peroxidase-like catalytic activity and its biosensing application
Autor: | Shouzhuo Yao, Meiling Liu, Cuiyan Wu, Youyu Zhang, Qiujun Lu, Jiali Hu, Haitao Li |
---|---|
Rok vydání: | 2019 |
Předmět: |
Biocompatibility
education Metal Nanoparticles Nanoparticle chemistry.chemical_element Nanotechnology Germanium Biosensing Techniques 02 engineering and technology 01 natural sciences Horseradish peroxidase Catalysis Analytical Chemistry chemistry.chemical_classification biology Benzidines Biomolecule Osmolar Concentration 010401 analytical chemistry Temperature technology industry and agriculture Hydrogen Peroxide Hydrogen-Ion Concentration Photothermal therapy 021001 nanoscience & nanotechnology Uric Acid 0104 chemical sciences Peroxidases chemistry Ionic strength biology.protein Colorimetry 0210 nano-technology Biosensor |
Zdroj: | Talanta. 195:407-413 |
ISSN: | 0039-9140 |
Popis: | In recent years, germanium nanoparticles (Ge NPs) have attracted considerable research attention because of its excellent electrical and optical properties. However, it is mainly applied in the field of solar cells, photodetectors and photothermal therapy. Actually, Ge NPs have the advantages of low toxicity and excellent biocompatibility, which means that they have great potential in biosensing applications. However, there are few related reports. In this paper, we discovered that Ge NPs have the intrinsic peroxidase-like catalytic activity and can significantly accelerate the oxidation of 3,3,5,5-tetramethylbenzidine (TMB) by H2O2 for the first time. Compared with natural enzyme (horseradish peroxidase, HRP), Ge NPs maintain higher catalytic stability after treatment at different pH, temperature and ionic strength. Furthermore, a Ge NPs-based colorimetric platform was established for the first time to detect biomolecules with favourable sensitivity and selectivity, such as uric acid, glucose and choline. This work finds the new property of Ge NPs and extends its application in biosensing. |
Databáze: | OpenAIRE |
Externí odkaz: |