Calcium-dependent Ret activation by GDNF and neurturin
| Autor: | Masahide Takahashi, Yosuke Iwata, Keizo Horibe, Chika Nozaki, Robert D. Klein, Hideki Murakami, Toshihide Iwashita, Arnon Rosenthal, Naoya Asai |
|---|---|
| Rok vydání: | 1998 |
| Předmět: |
Cancer Research
medicine.medical_specialty Glial Cell Line-Derived Neurotrophic Factor Receptors endocrine system diseases Cellular differentiation Neurturin Nerve Tissue Proteins Neuroblastoma Neurotrophic factors Internal medicine Proto-Oncogene Proteins Genetics medicine Glial cell line-derived neurotrophic factor Tumor Cells Cultured Drosophila Proteins Humans Glial Cell Line-Derived Neurotrophic Factor Nerve Growth Factors Phosphorylation Molecular Biology biology Autophosphorylation Proto-Oncogene Proteins c-ret Receptor Protein-Tyrosine Kinases Cell Differentiation Cell biology Enzyme Activation Endocrinology biology.protein Calcium Signal transduction GDNF family of ligands |
| Zdroj: | Oncogene. 16(3) |
| ISSN: | 0950-9232 |
| Popis: | Glial cell line-derived neurotrophic factor (GDNF) and neurturin (NTN) define a new family of neurotrophic factors that play crucial roles in survival and differentiation of various neurons. Recent studies demonstrated that GDNF and NTN use a multicomponent receptor system in which glycosyl-phosphatidylinositol (GPI)-linked cell surface proteins and Ret receptor tyrosine kinase function as the ligand-binding and signalling components, respectively. In the present study, we investigated the role of Ca2+ ions for biochemical and biological activities of Ret because Ret has a unique structure of the extracellular domain with the cadherin-like motif. The results demonstrated that Ca2+ ions might be required for the complex formation of Ret and GDNF or NTN that induces Ret oligomerization and autophosphorylation. Full morphological differentiation of neuroblastoma cells by these neurotrophic factors was also Ca2+-dependent. These findings thus suggested that, in addition to GPI-linked cell surface proteins, Ca2+ ions are components of the signal transducing complex formed by Ret and GDNF protein family. |
| Databáze: | OpenAIRE |
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