A NBD-based simple but effective fluorescent pH probe for imaging of lysosomes in living cells
| Autor: | Li-Na Chen, Xiang-Jian Cao, Jin-Ting Liu, Xuan Zhang, Qiu-Rong Wu, Bao-Xiang Zhao, Jun-Ying Miao, Ming-Yu Chen |
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| Rok vydání: | 2016 |
| Předmět: |
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Molecular Intracellular pH Analytical chemistry 02 engineering and technology Crystallography X-Ray 010402 general chemistry Photochemistry 01 natural sciences Biochemistry Analytical Chemistry Ph probe chemistry.chemical_compound Electron transfer Morpholine Ultraviolet light Humans Environmental Chemistry Moiety Spectroscopy Fluorescent Dyes Oxadiazoles Optical Imaging Time-dependent density functional theory Hydrogen-Ion Concentration 021001 nanoscience & nanotechnology Fluorescence 0104 chemical sciences Microscopy Fluorescence chemistry Lysosomes 0210 nano-technology HeLa Cells |
| Zdroj: | Analytica Chimica Acta. 920:86-93 |
| ISSN: | 0003-2670 |
| DOI: | 10.1016/j.aca.2016.03.029 |
| Popis: | NBDlyso with lysosome-locating morpholine moiety has been developed as a high selective and sensitive fluorescent pH probe. This probe can respond to acidic pH (2.0-7.0) in a short time (less than 1 min) and not almost change after continuously illuminated for an extended period by ultraviolet light. The fluorescence intensity of NBDlyso enhanced 100-fold in acidic solution, with very good linear relationship (R(2) = 0.996). The pKa of probe NBDlyso is 4.10. Therefore, NBDlyso was used to detect lysosomal pH changes successfully. Besides, X-ray crystallography was used to verify the structure of NBDlyso, and the recognition mechanism involving photo-induced electron transfer was interpreted theoretically by means of DFT and TDDFT calculations skillfully when NBDlyso comes into play under the acidic condition. This probe showed good ability to sense pH change in living cell image. |
| Databáze: | OpenAIRE |
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