Differential effects of amyloid-beta 1-40 and 1-42 fibrils on 5-HT1A serotonin receptors in rat brain

Autor: Anne Bérod, Luc Zimmer, Alexia Daoust, François Liger, Frédéric Bonnefoi, Fabien Chauveau, Anne-Laure Morel, Mathieu Verdurand
Přispěvatelé: Radiopharmaceutical and Neurochemical Biomarkers Team (BioRaN), Centre de recherche en neurosciences de Lyon (CRNL), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Université Jean Monnet [Saint-Étienne] (UJM)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Université Jean Monnet [Saint-Étienne] (UJM)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Centre d'Etude et de Recherche Multimodal Et Pluridisciplinaire en imagerie du vivant (CERMEP - imagerie du vivant), Université de Lyon-Université de Lyon-CHU Grenoble-Hospices Civils de Lyon (HCL)-CHU Saint-Etienne-Université Jean Monnet [Saint-Étienne] (UJM)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes (UGA)
Rok vydání: 2015
Předmět:
0301 basic medicine
Male
Aging
[SDV.IB.IMA]Life Sciences [q-bio]/Bioengineering/Imaging
Hippocampus
Plaque
Amyloid
Striatum
Rats
Sprague-Dawley
chemistry.chemical_compound
0302 clinical medicine
General Neuroscience
Neurogenesis
Alzheimer's disease
Immunohistochemistry
3. Good health
Amyloid-beta peptides
MPPF
Serotonin
Positron emission tomography
medicine.medical_specialty
Amyloid beta
Biology
Serotonergic
Injections
03 medical and health sciences
Alzheimer Disease
Internal medicine
medicine
Animals
Cognitive Dysfunction
5-HT receptor
Amyloid beta-Peptides
Corpus Striatum
Peptide Fragments
Rats
Disease Models
Animal
030104 developmental biology
Endocrinology
nervous system
chemistry
Positron-Emission Tomography
biology.protein
5-HT1A receptors
Neurology (clinical)
Geriatrics and Gerontology
Receptors
Serotonin
5-HT1
030217 neurology & neurosurgery
Developmental Biology
Zdroj: Neurobiology of Aging
Neurobiology of Aging, Elsevier, 2016, 40, pp.11-21. ⟨10.1016/j.neurobiolaging.2015.12.008⟩
ISSN: 1558-1497
0197-4580
DOI: 10.1016/j.neurobiolaging.2015.12.008⟩
Popis: International audience; Evidence accumulates suggesting a complex interplay between neurodegenerative processes and serotonergic neurotransmission. We have previously reported an overexpression of serotonin 5-HT1A receptors (5-HT1AR) after intrahippocampal injections of amyloid-beta 1–40 (Aβ40) fibrils in rats. This serotonergic reactivity paralleled results from clinical positron emission tomography studies with [18F]MPPF revealing an overexpression of 5-HT1AR in the hippocampus of patients with mild cognitive impairment. Because Aβ40 and Aβ42 isoforms are found in amyloid plaques, we tested in this study the hypothesis of a peptide- and region-specific 5-HT1AR reactivity by injecting them, separately, into the hippocampus or striatum of rats. [18F]MPPF in vitro autoradiography revealed that Aβ40 fibrils, but not Aβ42, were triggering an overexpression of 5-HT1AR in the hippocampus and striatum of rat brains after 7 days. Immunohistochemical approaches targeting neuronal precursor cells, mature neurons, and astrocytes showed that Aβ42 fibrils caused more pathophysiological damages than Aβ40 fibrils. The mechanisms of Aβ40 fibrils–induced 5-HT1AR expression remains unknown, but hypotheses including neurogenesis, glial expression, and axonal sprouting are discussed.
Databáze: OpenAIRE
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