Toxicity of poly-dispersed single-walled carbon nanotubes on bone marrow derived Hematopoietic Stem and Progenitor Cells
Autor: | Md. Babu Mia, Rajiv K. Saxena |
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Jazyk: | angličtina |
Rok vydání: | 2021 |
Předmět: |
Programmed cell death
HSPCs Lineage negative stem cell-enriched cells obtained after negative selection of bone marrow cells HSPC(dp) SCA-1+ cKit+ cell population within HSPCs Lymphoid and myeloid differentiation Health Toxicology and Mutagenesis medicine.medical_treatment Carbon nanotubes Caspase 3 AF-SWCNTs Acid-functionalized single-walled carbon nanotubes Toxicology Applied Microbiology and Biotechnology Article RA1190-1270 medicine Progenitor cell ComputingMethodologies_COMPUTERGRAPHICS Chemistry ROS Cell cycle HSC Hematopoietic stem cells Caspase Cell biology FAF-SWCNTs Fluorescence tagged AF-SWCNTs Haematopoiesis Cytokine medicine.anatomical_structure Toxicology. Poisons CNTs Carbon Nanotubes Bone marrow Stem cell Hematopoietic stem cells ROS Reactive Oxygen Species |
Zdroj: | Current Research in Toxicology, Vol 2, Iss, Pp 82-92 (2021) Current Research in Toxicology |
Popis: | Graphical abstract Highlights • AF-SWCNT administration caused decreased recovery of viable cells from bone marrow. • AF-SWCNTs induced cell death, ROS production and downregulated Caspase genes. • Cytokines induced differentiation of HSPCs to immune cells was also reduced. This study has explored the effect of acid-functionalized single-walled carbon nanotubes (AF-SWCNTs) on Hematopoietic Stem and Progenitor Cell (HSPCs) in mouse bone marrow. Administration of AF-SWCNTs induced a significant decline in the live-cell recovery from bone marrow. Lin-negative Stem cell enriched HSPCs internalized AF-SWCNTs that remained localized in cytoplasmic areas. Incubation of HSPCs with AF-SWCNTs resulted in induction of cell death, inhibition of cell cycle, and induction of reactive oxygen species (ROS) as well as the expression of Caspase 3, 7 and 9 enzymes. In vitro culture with a cytokine cocktail (SCF, GM-CSF, IL3, IL6, IL7) induced differentiation of HSPCs into lymphocytes and myeloid cells, that was inhibited in presence of AF-SWCNTs. Relative recoveries of lymphocytes specifically B lymphocytes, was significantly reduced by AF-SWCNT-treatment, whereas the relative recovery of myeloid cells remained unaltered. These results suggest that AF-SWCNTs have significant toxic effects on HSPCs and differentially suppress the ontogeny of lymphoid and myeloid cells. |
Databáze: | OpenAIRE |
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