Systematic expression alteration analysis of master reprogramming factor OCT4 and its three pseudogenes in human cancer and their prognostic outcomes
| Autor: | Subbroto Kumar Saha, Yeojin Jeong, Ssang-Goo Cho, Cho Sungha |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Pseudogene lcsh:Medicine Computational biology Biology Article Kruppel-Like Factor 4 03 medical and health sciences 0302 clinical medicine Breast cancer Mutation Rate SOX2 Cancer stem cell Neoplasms medicine Humans RNA Messenger Induced pluripotent stem cell lcsh:Science reproductive and urinary physiology Multidisciplinary fungi lcsh:R Cancer Cellular Reprogramming Prognosis medicine.disease Survival Analysis Gene Expression Regulation Neoplastic 030104 developmental biology KLF4 030220 oncology & carcinogenesis Mutation embryonic structures Human genome lcsh:Q biological phenomena cell phenomena and immunity Octamer Transcription Factor-3 Pseudogenes |
| Zdroj: | Scientific Reports, Vol 8, Iss 1, Pp 1-19 (2018) Scientific Reports |
| ISSN: | 2045-2322 |
| DOI: | 10.1038/s41598-018-33094-7 |
| Popis: | OCT4 is a master transcription factor that regulates the pluripotency of pluripotent stem cells and cancer stem cells along with other factors, including SOX2, KLF4, and C-MYC. Three different transcripts, OCT4A, OCT4B, and OCT4B1, are known to be generated by alternative splicing and eight OCT4 pseudogenes have been found in the human genome. Among them, we examined OCT4 and three pseudogenes (POU5F1P1, POU5F1P3, and POU5F1P4) because of their high expression possibility in cancer. In addition, previous studies indicated that OCT4 expression is augmented in cervical cancer and associated with poor prognosis, whereas OCT4 is down-regulated and correlated with good clinical outcomes in breast cancer. Because of these conflicting reports, we systematically evaluated whether expression of OCT4 and its pseudogenes can serve as oncogenic markers in various human cancers using the Oncomine database. Moreover, copy number alterations and mutations in OCT4 gene and its pseudogenes were analyzed using cBioPortal and the relationship between expression of OCT4 and pseudogenes and survival probability of cancer patients were explored using Kaplan-Meier plotter, OncoLnc, PROGgeneV2, and PrognoScan databases. Multivariate survival analysis was further conducted to determine the risk of the expression of the occurrence of OCT4 and its pseudogenes on certain cancer types using data from the Kaplan-Meier plotter. Overall, an association between expression of OCT4 and pseudogenes and cancer prognosis were established, which may serve as a therapeutic target for various human cancers. |
| Databáze: | OpenAIRE |
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