Identification and antigiardial activity of biocompounds produced in the Ganoderma lipsiense mycelium in submerged fermentation

Autor:	Juliane Araújo Greinert Goulart, Tania Maria Costa, Juliana Lenzi, Débora de Oliveira, Hercílio Higino da Silva Filho, Lorena Benathar Ballod Tavares, Michele Debiasi Alberton
Rok vydání:	2020
Předmět:	congenital hereditary and neonatal diseases and abnormalities Traditional medicine Giardia duodenalis Organic Chemistry Identification (biology) Plant Science Biology Ganoderma lipsiense Biochemistry Antiparasitic agent Submerged fermentation Mycelium Analytical Chemistry
Zdroj:	Natural Product Research. 35:5530-5534
ISSN:	1478-6427 1478-6419
DOI:	10.1080/14786419.2020.1793146
Popis:	Natural antiparasitic agents are used as a strategy for advances in the treatment of neglected tropical diseases (NTDs), such as giardiasis. The present paper identified the compounds produced by Ganoderma lipsiense mycelium grown in submerged fermentation and investigated the antigiardial activity potential of extracts in vitro. G. lipsiense mycelium crude extract (CE) was obtained by the maceration process and after purification in column, thirty compounds were identified by gas chromatography - mass spectrometry (GC-MS) in hexane sub-fraction (HSF). To determine the antigiardial potential, different parts of the purified CE have been evaluated, where hexane (HEXf) and dichloromethane (DCMf) fractions showed in vitro reduction levels higher than 90% of the Giardia duodenalis trophozoites at 100 μg mL−1. This antigiardial activity was investigated aiming to explore the possibility of G. lipsiense mycelium extract as a novel antigiardial compound to the treatment of human giardiasis.
Databáze:	OpenAIRE
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