Evaluation of the enzyme-linked immunoelectrotransfer blot assay for diagnosis of neurocysticercosis in children
| Autor: | Francisco Aguilar-Rebolledo, Javier Torres, Roberto Carlos Garcia, Onofre Muñoz, Antonio Meza-Lucas, Antonio Enciso, Dolores Correa, Roberto Cedillo-Rivera |
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| Rok vydání: | 2002 |
| Předmět: |
Male
Pathology medicine.medical_specialty Adolescent Neurocysticercosis Immunoblotting Antibodies Helminth Enzyme-Linked Immunosorbent Assay Biology 03 medical and health sciences 0302 clinical medicine Cerebrospinal fluid Western blot Antigen 030225 pediatrics parasitic diseases Parenchyma Taenia solium medicine Humans Child medicine.diagnostic_test Blot medicine.drug_formulation_ingredient medicine.anatomical_structure Antigens Helminth Pediatrics Perinatology and Child Health Immunology Female Neurology (clinical) Subarachnoid space 030217 neurology & neurosurgery |
| Zdroj: | Journal of child neurology. 17(6) |
| ISSN: | 0883-0738 |
| Popis: | Neurocysticercosis is a common problem in developing countries, and it causes neurologic disorders in children. Immunodiagnosis with Taenia solium glycoproteins as an antigen has been validated in adults but not in children. The aim of this work was to evaluate a Taenia solium glycoproteins-based enzyme-linked immunoelectrotransfer blot assay in children with neurocysticercosis. Twenty-five confirmed cases of neurocysticercosis and 50 healthy children from the same community were included. The test had a sensitivity of 72% and a specificity of 96%. Sensitivity was higher (100%) in cases with multiple cysts and in multiple sites. Sensitivity was higher when cysts were in parenchyma (86%) than when they were in the subarachnoid space. The most frequently recognized proteins were 24, 39 to 42, and 50 kDa. Diagnosis was more efficient in serum than in cerebrospinal fluid. Western blot is a reliable method for serologic diagnosis of neurocysticercosis in children. Multiple cysts and infections in multiple sites elicited a stronger immune response. (J Child Neurol 2002;17:416-420). |
| Databáze: | OpenAIRE |
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