Pairing Binge Drinking and a High-Fat Diet in Adolescence Modulates the Inflammatory Effects of Subsequent Alcohol Consumption in Mice

Autor: Jorge Manzanares, Sandra Montagud-Romero, Francisco Navarrete, José Miñarro, Macarena Gonzalez-Portilla, Ani Gasparyan, Marta Rodríguez-Arias
Přispěvatelé: Plan Nacional sobre Drogas (España), Ministerio de Sanidad, Servicios Sociales e Igualdad (España), Generalitat Valenciana, Instituto de Salud Carlos III, European Commission
Rok vydání: 2021
Předmět:
Male
0301 basic medicine
Chemokine CXCL1
Self Administration
Binge drinking
Alcohol
Striatum
Gut flora
Mice
chemistry.chemical_compound
0302 clinical medicine
Biology (General)
Binge
Spectroscopy
biology
alcohol
Microbiota
digestive
oral
and skin physiology
Age Factors
food and beverages
General Medicine
Computer Science Applications
Chemistry
High-fat diet
high-fat diet
Cytokines
binge
medicine.symptom
medicine.medical_specialty
Alcohol Drinking
QH301-705.5
Inflammation
Diet
High-Fat
digestive system
Article
Catalysis
Inorganic Chemistry
03 medical and health sciences
Internal medicine
Animals
Outbred Strains
microbiota
medicine
Animals
Obesity
Physical and Theoretical Chemistry
QD1-999
Molecular Biology
Neuroinflammation
Ethanol
Interleukin-6
business.industry
Organic Chemistry
biochemical phenomena
metabolism
and nutrition
biology.organism_classification
medicine.disease
binge drinking
cytokines
stomatognathic diseases
030104 developmental biology
Endocrinology
chemistry
inflammation
bacteria
business
030217 neurology & neurosurgery
Zdroj: International Journal of Molecular Sciences
Volume 22
Issue 10
Digital.CSIC. Repositorio Institucional del CSIC
instname
Zaguán. Repositorio Digital de la Universidad de Zaragoza
International Journal of Molecular Sciences, Vol 22, Iss 5279, p 5279 (2021)
ISSN: 1422-0067
DOI: 10.3390/ijms22105279
Popis: This article belongs to the Special Issue Gut Microbiota and Immunity.
Alcohol binge drinking (BD) and poor nutritional habits are two frequent behaviors among many adolescents that alter gut microbiota in a pro-inflammatory direction. Dysbiotic changes in the gut microbiome are observed after alcohol and high-fat diet (HFD) consumption, even before obesity onset. In this study, we investigate the neuroinflammatory response of adolescent BD when combined with a continuous or intermittent HFD and its effects on adult ethanol consumption by using a self-administration (SA) paradigm in mice. The inflammatory biomarkers IL-6 and CX3CL1 were measured in the striatum 24 h after BD, 3 weeks later and after the ethanol (EtOH) SA. Adolescent BD increased alcohol consumption in the oral SA and caused a greater motivation to seek the substance. Likewise, mice with intermittent access to HFD exhibited higher EtOH consumption, while the opposite effect was found in mice with continuous HFD access. Biochemical analyses showed that after BD and three weeks later, striatal levels of IL-6 and CX3CL1 were increased. In addition, in saline-treated mice, CX3CL1 was increased after continuous access to HFD. After oral SA procedure, striatal IL-6 was increased only in animals exposed to BD and HFD. In addition, striatal CX3CL1 levels were increased in all BD- and HFD-exposed groups. Overall, our findings show that adolescent BD and intermittent HFD increase adult alcohol intake and point to neuroinflammation as an important mechanism modulating this interaction.
This research was funded by Spanish Ministry of Health, Social Affairs and Equality, Government Delegation for the National Drugs Plan (2018/013 to MRA and PNSD 2016I016 to JMa); Generalitat Valenciana, Conselleria de Educación, Dirección General de Universidades, Grupos de Investigación de excelencia, PROMETEOII/2018/132 to JMi; Ministerio de Economía y Competitividad’ (FIS, PI14/00438) to JMa. Instituto de Salud Carlos III, Red de Trastornos Adictivos (RD16/0017/0007 to JMi) and Unión Europea, Fondos FEDER “una manera de hacer Europa”.
Databáze: OpenAIRE
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