Heparinase I (Neutralase) Reversal of Systemic Anticoagulation
| Autor: | King C. Lee, Joseph J. Zimmermann, Luis G. Michelsen, Mi Kyoung Lee, Jerrold H. Levy, Mutsuhito Kikura, Fania Szlam |
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| Rok vydání: | 1996 |
| Předmět: |
Whole Blood Coagulation Time
medicine.drug_class Pharmacology Dogs medicine Animals Humans Anesthesia Drug Interactions Protamines Specific enzyme Polysaccharide-Lyases Heparinase Dose-Response Relationship Drug biology Heparin business.industry Anticoagulant Hemodynamics Anticoagulants Heparin Antagonists Protamine Heparin lyase Anesthesiology and Pain Medicine Heparin Lyase Immunology biology.protein Systemic anticoagulation Heparinase I business medicine.drug |
| Zdroj: | Anesthesiology. 85:339-346 |
| ISSN: | 0003-3022 |
| Popis: | Background Protamine causes multiple adverse reactions. Heparinase I, a specific enzyme that inactivates heparin, is a possible alternative to protamine. In this study, the authors examined the efficacy of heparinase I to reverse heparin-induced anticoagulation in vitro and compared heparinase I to protamine as an antagonist of heparin-induced anticoagulation in dogs. Methods In the in vitro study, blood was obtained from the extracorporeal circuits of 12 patients, and activated clotting times were determined after adding different concentrations of heparinase I. In the in vivo study, 24 anesthetized dogs received 300 units/kg heparin injected intravenously for 5 s, then 10 min later, 3.9 mg/kg protamine, 5-41 micrograms/kg heparinase I, or the vehicle (n = 4/group) were administered intravenously, and activated clotting times and hemodynamics were measured. Results In the in vitro study, heparin concentrations of 3.3 +/- 1.0 (mean +/- SD) units/ml (approximately 0.033 mg/ml; n = 12) were reversed in the blood of patients by heparinase I at concentrations > 0.490 microgram/ml. In the canine study, heparinase at all doses studied and protamine effectively reversed the anticoagulating effects of heparin within 10 min of administration. Protamine produced adverse hemodynamic effects, whereas heparinase or its vehicle produced no significant change in arterial pressure. Conclusion Both heparinase I and protamine effectively reversed heparin anticoagulation. However, as opposed to protamine, heparinase I did not produce any significant hemodynamic changes when given as a bolus to dogs. |
| Databáze: | OpenAIRE |
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