Genetic analysis of Cln/Cdc28 regulation of cell morphogenesis in budding yeast
| Autor: | Benjamin K. Benton, Frederick R. Cross, Arthur H. Tinkelenberg, S. D. Plump, D. Jean |
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| Jazyk: | angličtina |
| Rok vydání: | 1993 |
| Předmět: |
Saccharomyces cerevisiae Proteins
Saccharomyces cerevisiae Genes Fungal Restriction Mapping Biology medicine.disease_cause General Biochemistry Genetics and Molecular Biology GTP Phosphohydrolases Fungal Proteins Cyclins medicine Gene family Molecular Biology Gene Genetics Cyclin-dependent kinase 1 Mutation General Immunology and Microbiology Cell morphogenesis General Neuroscience Cell Cycle GTPase-Activating Proteins Genetic Complementation Test Cell cycle biology.organism_classification Cell biology Mutagenesis Insertional GTP Phosphohydrolase Activators CDC28 Protein Kinase S cerevisiae Cytokinesis Research Article |
| Popis: | The CLN1, CLN2 and CLN3 gene family of G1-acting cyclin homologs of Saccharomyces cerevisiae is functionally redundant: any one of the three Cln proteins is sufficient for activation of Cdc28p protein kinase activity for cell cycle START. The START event leads to multiple processes (including DNA replication and bud emergence); how Cln/Cdc28 activity activates these processes remains unclear. CLN3 is substantially different in structure and regulation from CLN1 and CLN2, so its functional redundancy with CLN1 and CLN2 is also poorly understood. We have isolated mutations that alter this redundancy, making CLN3 insufficient for cell viability in the absence of CLN1 and CLN2 expression. Mutations causing phenotypes specific for the cell division cycle were analyzed in detail. Mutations in one gene result in complete failure of bud formation, leading to depolarized cell growth. This gene was identified as BUD2, previously described as a non-essential gene required for proper bud site selection but not required for budding and viability. Bud2p is probably the GTPase-activating protein for Rsr1p/Bud1p [Park, H., Chant, I. and Herskowitz, I. (1993) Nature, 365, 269-274]; we find that Rsr1p is required for the bud2 lethal phenotype. Mutations in two other genes (ERC10 and ERC19) result in a different morphogenetic defect: failure of cytokinesis resulting in the formation of long multinucleate tubes. These results suggest direct regulation of diverse aspects of bud morphogenesis by Cln/Cdc28p activity. |
| Databáze: | OpenAIRE |
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