Mrc1 is required for normal progression of replication forks throughout chromatin in S. cerevisiae
| Autor: | Christopher J. Viggiani, Shawn J. Szyjka, Oscar M. Aparicio |
|---|---|
| Rok vydání: | 2005 |
| Předmět: |
DNA Replication
Saccharomyces cerevisiae Proteins Eukaryotic DNA replication Cell Cycle Proteins Saccharomyces cerevisiae Biology Pre-replication complex 03 medical and health sciences 0302 clinical medicine Minichromosome maintenance Control of chromosome duplication Molecular Biology 030304 developmental biology Genetics 0303 health sciences DNA replication DNA Helicases Nuclear Proteins Cell Biology Chromatin DNA-Binding Proteins Mutation Replisome Origin recognition complex 030217 neurology & neurosurgery |
| Zdroj: | Molecular cell. 19(5) |
| ISSN: | 1097-2765 |
| Popis: | Mrc1 associates with replication forks, where it transmits replication stress signals and is required for normal replisome pausing in response to nucleotide depletion. Mrc1 also plays a poorly understood role in DNA replication, which appears distinct from its role in checkpoint signaling. Here, we demonstrate that Mrc1 functions constitutively to promote normal replication fork progression. In mrc1Delta cells, replication forks proceed slowly throughout chromatin, rather than being specifically defective in pausing and progression through loci that impede fork progression. Analysis of genetic interactions with Rrm3, a DNA helicase required to resolve paused forks, indicates that Mrc1 checkpoint signaling is dispensable for the resolution of stalled replication forks and suggests that replication forks lacking Mrc1 create DNA damage that must be repaired by Rrm3. These findings elucidate a central role for Mrc1 in normal replisome function, which is distinct from its role as a checkpoint mediator, but nevertheless critical to genome stability. |
| Databáze: | OpenAIRE |
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