Proliferation and maturation of microvessels in arteriovenous malformations - expression patterns of angiogenic and cell cycle-dependent factors
| Autor: | Sandrine Florquin, Onno J. de Boer, Allard C. van der Wal, Lorine B. Meijer-Jorna, Peter Teeling, Chantal M.A.M. van der Horst, Chris M. van der Loos |
|---|---|
| Přispěvatelé: | Other departments, Pathology, ACS - Amsterdam Cardiovascular Sciences, AII - Amsterdam institute for Infection and Immunity, Other Research, Plastic, Reconstructive and Hand Surgery |
| Jazyk: | angličtina |
| Rok vydání: | 2012 |
| Předmět: |
Male
Vascular Endothelial Growth Factor A Pathology medicine.medical_specialty Histology Angiogenesis Cell Cycle Proteins Dermatology Pathology and Forensic Medicine Angiopoietin Hemangioma Arteriovenous Malformations chemistry.chemical_compound Translational research [ONCOL 3] medicine Angiopoietin-1 Humans Granuloma Pyogenic Microvessel Skin Neovascularization Pathologic business.industry Caspase 3 Vascular malformation Cell Cycle Cell cycle medicine.disease Immunohistochemistry Vascular endothelial growth factor chemistry Microvessels Female Tumor Suppressor Protein p53 business Immunostaining |
| Zdroj: | Journal of Cutaneous Pathology, 39, 6, pp. 610-20 Journal of Cutaneous Pathology, 39, 610-20 Journal of cutaneous pathology, 39(6), 610-620. Wiley-Blackwell |
| ISSN: | 0303-6987 |
| Popis: | Background: Areas of microvascular proliferation have been observed in a subpopulation of symptomatic congenital vascular malformations later in life. We investigated whether this angiogenic response is followed by a stage of maturation. Methods: Resections of vascular malformations (n = 15), infantile hemangiomas (IHs) (n = 8) and pyogenic granulomas (PGs) (n = 5) were studied. Histopathologically, all lesions were screened for presence of foci of immature and/or mature microvessels. These areas were further studied immunohistochemically for differential expression of several angiogenic factors, cell cycle-dependent proteins, p53 and active caspase3. Immunostains were scored semiquantitatively. Results: Immature microvessel areas were present in five vascular malformations (all of the arteriovenous type), five IHs and five PGs; these lesions also contained transitions between immature and mature microvessels. Conglomerates of mature microvessels were found in 19 cases (6 vascular malformations, 5 PGs and 8 IHs). Expression of vascular endothelial growth factor-A, angiopoietin-1, Ki-67, p16 and p21/27 ratios were overall significantly lower in mature areas than in immature areas including those in vascular malformations. P53 and caspase3 expression was scarce in all lesions. Conclusions: Microvascular areas in vascular malformations appear to follow the same pattern of vascular proliferation and maturation as seen in other microvascular lesions of skin and soft tissue. Meijer-Jorna LB, van der Loos CM, Teeling P, de Boer OJ, Florquin S, van der Horst CMAM, van der Wal AC. Proliferation and maturation of microvessels in arteriovenous malformations expression patterns of angiogenic and cell cycle-dependent factors |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|