A conserved filamentous assembly underlies the structure of the meiotic chromosome axis
| Autor: | Qiaozhen Ye, Madison K Lehmer, Scott C. Rosenberg, Iracema Caballero, Isabel Usón, Amy J. MacQueen, Götz Hagemann, Kevin D. Corbett, Alan M V West, Franz Herzog, Sarah N. Ur |
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| Přispěvatelé: | National Institutes of Health (US), National Science Foundation (US), Ministerio de Ciencia e Innovación (España), Ministerio de Economía y Competitividad (España), Ludwig Institute for Cancer Research (US) |
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
Mouse
Chromosomal Proteins Non-Histone Structural Biology and Molecular Biophysics Arabidopsis S. cerevisiae Cell Cycle Proteins Haploidy Mass Spectrometry Scattering Double-Stranded Mice 0302 clinical medicine Gene expression Protein Interaction Mapping structural biology Scattering Radiation DNA Breaks Double-Stranded Biology (General) Coiled coil Recombination Genetic 0303 health sciences meiotic recombination Radiation Synaptonemal Complex General Neuroscience Nuclear Proteins General Medicine Zygosaccharomyces rouxii Chromosomes and Gene Expression 3. Good health Cell biology Chromosomal Proteins HORMAD protein Meiosis meiotic chromosome axis Medicine Recombination Biotechnology Research Article chromosomes Saccharomyces cerevisiae Proteins QH301-705.5 Science 1.1 Normal biological development and functioning Meiotic chromosome Saccharomyces cerevisiae Biology General Biochemistry Genetics and Molecular Biology Chromosomes 03 medical and health sciences Genetic Protein Domains Underpinning research Two-Hybrid System Techniques Genetics molecular biophysics coiled-coil Animals mouse 030304 developmental biology General Immunology and Microbiology DNA Breaks fungi Zygosaccharomyces Chromosome Non-Histone Kinetics Structural biology A. thaliana Mutation gene expression Generic health relevance Biochemistry and Cell Biology Other Homologous recombination 030217 neurology & neurosurgery Synchrotrons |
| Zdroj: | eLife Digital.CSIC. Repositorio Institucional del CSIC instname eLife, Vol 8 (2019) |
| ISSN: | 2050-084X 2015-6421 |
| Popis: | The meiotic chromosome axis plays key roles in meiotic chromosome organization and recombination, yet the underlying protein components of this structure are highly diverged. Here, we show that 'axis core proteins' from budding yeast (Red1), mammals (SYCP2/SYCP3), and plants (ASY3/ASY4) are evolutionarily related and play equivalent roles in chromosome axis assembly. We first identify 'closure motifs' in each complex that recruit meiotic HORMADs, the master regulators of meiotic recombination. We next find that axis core proteins form homotetrameric (Red1) or heterotetrameric (SYCP2:SYCP3 and ASY3:ASY4) coiled-coil assemblies that further oligomerize into micron-length filaments. Thus, the meiotic chromosome axis core in fungi, mammals, and plants shares a common molecular architecture, and likely also plays conserved roles in meiotic chromosome axis assembly and recombination control. SU acknowledges past support from the UC San Diego Molecular Biophysics Training Grant (National Institutes of Health T32 GM008326), and current support from the National Science Foundation (Graduate Research Fellowship). IU and IC are supported by grants BIO2015-64216-P and MDM2014-0435 (the Spanish Ministry of Science, Innovation and Universities). AJM acknowledges support from the National Institutes of Health (R15 GM116109). KDC acknowledges past support from the Ludwig Institute for Cancer Research and the National Institutes of Health (R01 GM104141). KDC and FH acknowledge joint support from the Human Frontiers Science Program (RGP0008/2015). |
| Databáze: | OpenAIRE |
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