The leucine-NH4+ uptake regulator Any1 limits growth as part of a general amino acid control response to loss of La protein by fission yeast
| Autor: | James R. Iben, Vera A. Cherkasova, Alan C. Kessler, Richard J. Maraia, Kevin J. Pridham |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Arrestins
Mutant TRNA processing Gene Expression Yeast and Fungal Models Biochemistry Schizosaccharomyces Pombe Gene expression Amino Acids chemistry.chemical_classification Multidisciplinary biology Chemistry Organic Compounds Eukaryota RNA-Binding Proteins Genomics Cell biology Amino acid Nucleic acids Experimental Organism Systems Transfer RNA Physical Sciences Amino Acid Analysis Medicine Saccharomyces Cerevisiae Leucine Transcriptome Analysis Research Article Science Saccharomyces cerevisiae Genes Fungal Research and Analysis Methods Saccharomyces Model Organisms Schizosaccharomyces Genetics Non-coding RNA Molecular Biology Techniques Molecular Biology Molecular Biology Assays and Analysis Techniques Gene Expression Profiling Organic Chemistry Organisms Fungi Chemical Compounds Biology and Life Sciences Proteins Computational Biology biology.organism_classification Genome Analysis Yeast Amino Acid Metabolism Metabolism Aliphatic Amino Acids Schizosaccharomyces pombe Mutation Animal Studies RNA Schizosaccharomyces pombe Proteins |
| Zdroj: | PLoS ONE PLoS ONE, Vol 16, Iss 6, p e0253494 (2021) |
| ISSN: | 1932-6203 |
| Popis: | The sla1+ gene of Schizosachharoymces pombe encodes La protein which promotes proper processing of precursor-tRNAs. Deletion of sla1 (sla1Δ) leads to disrupted tRNA processing and sensitivity to target of rapamycin (TOR) inhibition. Consistent with this, media containing NH4+ inhibits leucine uptake and growth of sla1Δ cells. Here, transcriptome analysis reveals that genes upregulated in sla1Δ cells exhibit highly significant overalp with general amino acid control (GAAC) genes in relevant transcriptomes from other studies. Growth in NH4+ media leads to additional induced genes that are part of a core environmental stress response (CESR). The sla1Δ GAAC response adds to evidence linking tRNA homeostasis and broad signaling in S. pombe. We provide evidence that deletion of the Rrp6 subunit of the nuclear exosome selectively dampens a subset of GAAC genes in sla1Δ cells suggesting that nuclear surveillance-mediated signaling occurs in S. pombe. To study the NH4+-effects, we isolated sla1Δ spontaneous revertants (SSR) of the slow growth phenotype and found that GAAC gene expression and rapamycin hypersensitivity were also reversed. Genome sequencing identified a F32V substitution in Any1, a known negative regulator of NH4+-sensitive leucine uptake linked to TOR. We show that 3H-leucine uptake by SSR-any1-F32V cells in NH4+-media is more robust than by sla1Δ cells. Moreover, F32V may alter any1+ function in sla1Δ vs. sla1+ cells in a distinctive way. Thus deletion of La, a tRNA processing factor leads to a GAAC response involving reprogramming of amino acid metabolism, and isolation of the any1-F32V rescuing mutant provides an additional specific link. |
| Databáze: | OpenAIRE |
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