Quantitative determination of diterpenoid alkaloid Fuziline by hydrophilic interaction liquid chromatography (HILIC)–electrospray ionization mass spectrometry and its application to pharmacokinetic study in rats
| Autor: | Yunxi Zhong, Jianguo Sun, Guangji Wang, Fengyi Zhang, Jing-Han Liu, Ying Peng |
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| Rok vydání: | 2013 |
| Předmět: |
Male
Spectrometry Mass Electrospray Ionization Electrospray Electrospray ionization Liquid-Liquid Extraction Clinical Biochemistry Mass spectrometry Biochemistry Analytical Chemistry Rats Sprague-Dawley Matrix (chemical analysis) Alkaloids Drug Stability Limit of Detection Liquid chromatography–mass spectrometry Animals Selected ion monitoring Detection limit Aconitum Chromatography Plant Extracts Chemistry Hydrophilic interaction chromatography Reproducibility of Results Cell Biology General Medicine Rats Female Diterpenes Hydrophobic and Hydrophilic Interactions Chromatography Liquid |
| Zdroj: | Journal of Chromatography B. :55-60 |
| ISSN: | 1570-0232 |
| DOI: | 10.1016/j.jchromb.2012.11.017 |
| Popis: | A rapid, sensitive and specific hydrophilic interaction liquid chromatography coupled to electrospray ionization mass spectrometric (HILIC-MS) method for the quantification of Fuziline (15α-Hydroxyneoline) in rat plasma was developed and validated. After liquid-liquid extraction with ethyl acetate, Fuziline and Guanfu base A (internal standard) were separated with HILIC Chrom Matrix HP amide column (5μm, 10cm×3.0mm I.D.) with isocratic elution at a flow-rate of 0.2mL/min. The analytes were detected by using an electrospray positive ionization mass spectrometry in the selected ion monitoring (SIM) mode. A good linear relationship was obtained in the concentration ranging from 1 to 1000ng/mL (R(2)=0.999) with the lower limit of quantification (LLOQ) at 1ng/mL and limit of detection (LOD) at 0.5ng/mL. The average recoveries of Fuziline in plasma at the concentrations of 2, 50, 1000ng/mL ranged from 68.2 to 69.9%. Intra- and inter-batch relative standard deviations ranged from 1.5 to 3.3% and 2.6 to 8.3%, respectively. Fuziline was stable under different sample storage and processing conditions except three-cycle freeze-thaw treatment at 2ng/mL. This method was successfully applied to the pharmacokinetic studies in Sprague-Dawley rats. The absolute bioavailability of Fuziline after oral administration 4mg/kg Fuziline in rats was 21.1±7.0%, with clearance rate at 1745.6±818.1mL/kg/h, and half-life at about 6.3±2.6h. |
| Databáze: | OpenAIRE |
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