Attenuation of Pseudomonas aeruginosa biofilm formation by Vitexin: A combinatorial study with azithromycin and gentamicin
Autor: | Prasenjit Rudrapaul, Manash C. Das, Priya Gupta, Padmani Sandhu, Prosun Tribedi, Yusuf Akhter, Utpal Chandra De, Surajit Bhattacharjee |
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Rok vydání: | 2016 |
Předmět: |
0301 basic medicine
030106 microbiology Vitexin Swarming motility Microbial Sensitivity Tests Azithromycin Biology medicine.disease_cause Article Microbiology Mice 03 medical and health sciences chemistry.chemical_compound Minimum inhibitory concentration Pyocyanin medicine Animals Apigenin Multidisciplinary Pseudomonas aeruginosa Biofilm Quorum Sensing Antimicrobial Anti-Bacterial Agents Quorum sensing 030104 developmental biology chemistry Biofilms Gentamicins |
Zdroj: | Scientific Reports |
ISSN: | 2045-2322 |
Popis: | Microbial biofilm are communities of surface-adhered cells enclosed in a matrix of extracellular polymeric substances. Extensive use of antibiotics to treat biofilm associated infections has led to the emergence of multiple drug resistant strains. Pseudomonas aeruginosa is recognised as a model biofilm forming pathogenic bacterium. Vitexin, a polyphenolic group of phytochemical with antimicrobial property, has been studied for its antibiofilm potential against Pseudomonas aeruginosa in combination with azithromycin and gentamicin. Vitexin shows minimum inhibitory concentration (MIC) at 260 μg/ml. It’s antibiofilm activity was evaluated by safranin staining, protein extraction, microscopy methods, quantification of EPS and in vivo models using several sub-MIC doses. Various quorum sensing (QS) mediated phenomenon such as swarming motility, azocasein degrading protease activity, pyoverdin and pyocyanin production, LasA and LasB activity of the bacteria were also evaluated. Results showed marked attenuation in biofilm formation and QS mediated phenotype of Pseudomonas aeruginosa in presence of 110 μg/ml vitexin in combination with azithromycin and gentamicin separately. Molecular docking of vitexin with QS associated LuxR, LasA, LasI and motility related proteins showed high and reasonable binding affinity respectively. The study explores the antibiofilm potential of vitexin against P. aeruginosa which can be used as a new antibiofilm agent against microbial biofilm associated pathogenesis. |
Databáze: | OpenAIRE |
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