An experimental study of shear-dependent human platelet adhesion and underlying protein-binding mechanisms in a cylindrical Couette system
| Autor: | Mehdi Behbahani, Linda Tran, Khosrow Mottaghy, Sabine Arlt-Körfer, Christian Waluga |
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| Rok vydání: | 2015 |
| Předmět: |
Blood Platelets
Materials science biology Drop (liquid) 0206 medical engineering Biomedical Engineering Thermodynamics Fibrinogen 02 engineering and technology Plasma protein binding 020601 biomedical engineering Shear rate 03 medical and health sciences 0302 clinical medicine Platelet Adhesiveness Von Willebrand factor Shear (geology) von Willebrand Factor biology.protein Shear stress Humans Platelet Carrier Proteins Couette flow 030217 neurology & neurosurgery |
| Zdroj: | Biomedizinische Technik. Biomedical engineering. 62(4) |
| ISSN: | 1862-278X |
| Popis: | Undesirable thrombotic reactions count among the most frequent and serious complications for patients who rely on the use of medical devices. To improve the design of medical devices, it is essential to develop a more precise understanding of platelet reactions. Clinical studies and experiments have shown a strong dependence of platelet deposition behavior on the flow. However, today the influence of hemodynamic parameters such as the shear rate on thrombotic reactions is not well understood. For the study of the shear-dependent mechanisms leading to the activation, adhesion and aggregation of platelets, a Couette flow system was used to investigate thrombocyte behavior with regard to well-defined flow conditions at shear-rate values between γ˙=400 $\dot \gamma = {\rm{400}}$ and 1400 1/s. Results were calculated for physiological temperature. It could be shown that the platelet adhesion density increased with increasing shear rates up to γ˙=800 1/s $\dot \gamma = {\rm{800 1/s}}$ and the adhesion pattern was homogeneous. At γ˙=800 1/s, $\dot \gamma = {\rm{800 1/s}},$ a sudden drop in platelet adhesion density occurred and platelets adhered in filaments. Fluorescence microscopy results of von Willebrand factor (vWF) confirm that a shear rate of γ˙=800 1/s $\dot \gamma = {\rm{800 1/s}}$ represents the threshold where a switch of the platelet-binding mechanism from fibrinogen-mediated to vWF-mediated platelet adhesion takes place. |
| Databáze: | OpenAIRE |
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