In vitrolipolysis tests on lipid nanoparticles: comparison between lipase/co-lipase and pancreatic extract
| Autor: | Stéphanie Chevrier, Frédéric Demarne, Vincent Jannin, Yann Chavant, Christophe Voutsinas, Cristina Bonferoni, Eleonora Dellera |
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| Rok vydání: | 2014 |
| Předmět: |
Swine
Lipolysis Dietary lipid Administration Oral Biological Availability Pharmaceutical Science Pancreatic Extracts Carboxylesterase Drug Discovery Solid lipid nanoparticle Animals Lipase Pharmacology chemistry.chemical_classification Gastrointestinal tract biology Organic Chemistry Hydrogen-Ion Concentration Lipids Bioavailability Drug Liberation Enzyme chemistry Biochemistry biology.protein Nanoparticles Digestion |
| Zdroj: | Drug Development and Industrial Pharmacy. 41:1582-1588 |
| ISSN: | 1520-5762 0363-9045 |
| DOI: | 10.3109/03639045.2014.972412 |
| Popis: | Solid lipid nanoparticles (SLNs) and nanostructured lipid carriers (NLC) are lipid nanocarriers aimed to the delivery of drugs characterized by a low bioavailability, such as poorly water-soluble drugs and peptides or proteins. The oral administration of these lipid nanocarriers implies the study of their lipolysis in presence of enzymes that are commonly involved in dietary lipid digestion in the gastrointestinal tract. In this study, a comparison between two methods was performed: on one hand, the lipase/co-lipase assay, commonly described in the literature to study the digestion of lipid nanocarriers, and on the other hand, the lipolysis test using porcine pancreatic extract and the pH-stat apparatus. This pancreatic extract contains both the pancreatic lipase and carboxyl ester hydrolase (CEH) that permit to mimic in a biorelevant manner the duodenal digestive lipolysis. The test was performed by means of a pH-stat apparatus to work at constant pH, 5.5 or 6.25, representing respectively the fasted or fed state pH conditions. The evolution of all acylglycerol entities was monitored during the digestion by sampling the reaction vessel at different time points, until 60 min, and the lipid composition of the digest was analyzed by gas chromatography. SLN and NLC systems obtained with long-chain saturated acylglycerols were rapidly and completely digested by pancreatic enzymes. The pH-stat titration method appears to be a powerful technique to follow the digestibility of these solid lipid-based nanoparticles. |
| Databáze: | OpenAIRE |
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