Interaction of a gene-specific transcription factor with the adenovirus major late promoter upstream of the TATA box region
| Autor: | Robert G. Roeder, Michele Sawadogo |
|---|---|
| Rok vydání: | 1985 |
| Předmět: |
Hot Temperature
Base Sequence Transcription Genetic TATA box Downstream promoter element Promoter Templates Genetic Biology Molecular biology General Biochemistry Genetics and Molecular Biology Upstream Stimulatory Factor Adenoviridae TAF1 DNA Viral Transcription Factor TFIID Deoxyribonuclease I Promoter Regions Genetic Transcription factor II B Transcription factor II A Transcription Factors |
| Zdroj: | Cell. 43:165-175 |
| ISSN: | 0092-8674 |
| DOI: | 10.1016/0092-8674(85)90021-2 |
| Popis: | A gene-specific transcription factor, called USF, has been partially purified from HeLa cell nuclear extracts. Addition of USF results in a 10 to 20 fold increase in transcription from the adenovirus major late promoter in an in vitro system reconstituted with transcription factors TFIIB, TFIID, TFIIE, and RNA polymerase II. Binding of USF to the promoter inhibits DNAase I cleavages over a 20 base pair region just upstream of the -45 to +35 region shown previously to interact with TFIID. More discriminating footprint analyses using methidiumpropyl-EDTA-Fe(II) as the cleaving agent indicate that USF interacts primarily with the small palindromic DNA sequence GGCCACGTGACC located between positions -63 and -52 of the major late promoter, while TFIID interacts primarily with a 10 base pair DNA region centered on the consensus TATA sequence. Dissociation rate measurements indicate a cooperative interaction between USF and TFIID when simultaneously bound to the promoter DNA. |
| Databáze: | OpenAIRE |
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