Comparative immunological recognition of proteins from New Guinea 'C' dengue virus type 2 prototype and from a dengue virus type 2 strain isolated in the State of Rio de Janeiro, Brazil
| Autor: | Carlos Roberto Alves, Josery R. Pantoja, Luzia Monteiro de Castro Côrtes, Ortrud Monika Barth |
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| Rok vydání: | 2003 |
| Předmět: |
Microbiology (medical)
Antigenicity lcsh:Arctic medicine. Tropical medicine lcsh:RC955-962 viruses Secondary infection Blotting Western lcsh:QR1-502 Enzyme-Linked Immunosorbent Assay Viral Nonstructural Proteins Dengue virus Biology medicine.disease_cause lcsh:Microbiology Virus Microbiology Epitopes medicine Animals Humans Viral Structural Proteins New Guinea NS3 Strain (chemistry) Flavivirus virus diseases New guinea Dengue Virus Hemagglutination Inhibition Tests biochemical phenomena metabolism and nutrition biology.organism_classification Virology dengue virus type 2 proteins antigenicity Rio de Janeiro Electrophoresis Polyacrylamide Gel Brazil geographic locations |
| Zdroj: | Memórias do Instituto Oswaldo Cruz, Volume: 98, Issue: 3, Pages: 331-334, Published: APR 2003 Memórias do Instituto Oswaldo Cruz., Vol 98, Iss 3, Pp 331-334 (2003) |
| ISSN: | 0074-0276 |
| DOI: | 10.1590/s0074-02762003000300007 |
| Popis: | The protein profiles of the New Guinea "C" dengue virus type 2 (DENV-2)prototype and those of a Brazilian DENV-2 isolated in the State of Rio de Janeiro in 1995 were compared. SDS-PAGE analysis showed that the virus from Rio de Janeiro expresses NS5 (93.0 kDa), NS3 (66.8 kDa) E (62.4 kDa) and NS1 (41.2 kDa) proteins differently from the New Guinea "C" virus. The immunoblot revealed specificity and antigenicity for the NS3 protein from DENV-2 Rio de Janeiro mainly in primary infections, convalescent cases, and in secondary infections in both cases and only antigenicity for E and NS1 proteins for both viruses in primary and secondary infections. |
| Databáze: | OpenAIRE |
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