Sedative and anesthetic activities of the essential oils of Hyptis mutabilis (Rich.) Briq. and their isolated components in silver catfish (Rhamdia quelen)
| Autor: | Quelen Iane Garlet, Lenise de Lima Silva, G.S. Dolci, Bernardo Baldisserotto, Solon Jonas Longhi, Berta Maria Heinzmann, Marilise Escobar Burger, Simone Cristina Benovit, Carlos Augusto Mallmann |
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| Rok vydání: | 2013 |
| Předmět: |
Physiology
Hyptis Biochemistry Terpene Toxicology GABA chemistry.chemical_compound Hypnotics and Sedatives Inflorescence General Pharmacology Toxicology and Pharmaceutics lcsh:QH301-705.5 Catfishes lcsh:R5-920 Traditional medicine biology General Neuroscience General Medicine (+)-1-Terpinen-4-ol Eugenol Sedation lcsh:Medicine (General) Sesquiterpenes medicine.drug Catfish GABA Agents medicine.drug_class Immunology Biophysics Silver catfish Fractionation Gas Chromatography-Mass Spectrometry Statistics Nonparametric (-)-Globulol Oils Volatile medicine Animals Mortality Anesthetics Analysis of Variance Chemotype Terpenes business.industry Biomedical Sciences Cell Biology biology.organism_classification Plant Leaves lcsh:Biology (General) chemistry Sedative Anesthetic business |
| Zdroj: | Brazilian Journal of Medical and Biological Research, Vol 46, Iss 9, Pp 771-779 (2013) Brazilian Journal of Medical and Biological Research, Volume: 46, Issue: 9, Pages: 771-779, Published: 18 SEP 2013 Brazilian Journal of Medical and Biological Research v.46 n.9 2013 Brazilian Journal of Medical and Biological Research Associação Brasileira de Divulgação Científica (ABDC) instacron:ABDC |
| ISSN: | 0100-879X |
| DOI: | 10.1590/1414-431x20133013 |
| Popis: | This study evaluated the sedative and anesthetic effects of the essential oils (EO) of Hyptis mutabilis (Rich.) Briq. and their isolated components on silver catfish (Rhamdia quelen). Quantitative chemical differences between the EOs obtained from leaves and inflorescences were verified, and a new chemotype rich in globulol was described. Although there were no significant differences in the time of induction for sedation and anesthesia between the EOs, only the leaf EO at 344 mg/L anesthetized all fish without side effects. Fractionation of the leaf EO was carried out by column chromatography. The isolated compounds [(+)-1-terpinen-4-ol and (-)-globulol] showed different activity from that detected for the leaf EO in proportional concentrations and similar sedation to a eugenol control at 10 mg/L. However, fish exposed to 1-terpinen-4-ol (3 and 10 mg/L) did not remain sedated for 30 min. Anesthesia was obtained with 83-190 mg/L globulol, but animals showed loss of mucus during induction and mortality at these concentrations. Synergism of the depressor effects was detected with the association of globulol and benzodiazepine (BDZ), compared with either drug alone. Fish exposed to BDZ or globulol+BDZ association showed faster recovery from anesthesia in water containing flumazenil, but the same did not occur with globulol. In conclusion, the use of globulol in aquaculture procedures should be considered only at sedative concentrations of 10 and 20 mg/L, and its mechanism of action seems not to involve the GABAA-BDZ system. |
| Databáze: | OpenAIRE |
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