Human Innate Lymphoid Cell Subsets Possess Tissue-Type Based Heterogeneity in Phenotype and Frequency
| Autor: | Florent Ginhoux, Eng Huat Tan, Muhammad Shabeer, Sonia Baig, Maria A. Curotto de Lafaille, Tony Kiat Hon Lim, Chan Weng Hoong, Si-Lin Koo, Paul Klenerman, Koh Dennis, Ming Hian Kam, Choong-Leong Tang, Joannah R. Fergusson, Yannick Simoni, Iain Beehuat Tan, Shawn Lim, Angela Takano, Ayako Kurioka, Evan W. Newell, Jerry Kok Yen Chan, Daniel Shao-Weng Tan, Michael G. Fehlings, Rosslyn Anicete, Naomi McGovern, Sriram Narayanan, Henry K.K. Tan, Alasdair Leslie, Alexander Chung Yaw Fui, Sue-Anne Ee Shiow Toh, Chiew Yee Loh, Henrik N. Kløverpris |
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| Rok vydání: | 2015 |
| Předmět: |
0301 basic medicine
Cell type Immunology Biology Article Flow cytometry 03 medical and health sciences 0302 clinical medicine Immune system medicine Immunology and Allergy Humans Mass cytometry Lymphocytes skin and connective tissue diseases Transcription factor medicine.diagnostic_test Innate lymphoid cell Flow Cytometry Phenotype Immunity Innate Lymphocyte Subsets Cell biology body regions 030104 developmental biology Infectious Diseases Tissue type 030215 immunology |
| Zdroj: | Immunity |
| ISSN: | 1097-4180 |
| Popis: | Animal models have highlighted the importance of innate lymphoid cells (ILCs) in multiple immune responses. However, technical limitations have hampered adequate characterization of ILCs in humans. Here, we used mass cytometry including a broad range of surface markers and transcription factors to accurately identify and profile ILCs across healthy and inflamed tissue types. High dimensional analysis allowed for clear phenotypic delineation of ILC2 and ILC3 subsets. We were not able to detect ILC1 cells in any of the tissues assessed, however, we identified intra-epithelial (ie)ILC1-like cells that represent a broader category of NK cells in mucosal and non-mucosal pathological tissues. In addition, we have revealed the expression of phenotypic molecules that have not been previously described for ILCs. Our analysis shows that human ILCs are highly heterogeneous cell types between individuals and tissues. It also provides a global, comprehensive, and detailed description of ILC heterogeneity in humans across patients and tissues. |
| Databáze: | OpenAIRE |
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