FBXO7 Immunoreactivity in alpha-Synuclein-Containing Inclusions in Parkinson Disease and Multiple System Atrophy
| Autor: | Renate K. Hukema, Ben A. Oostra, Ping Pin Zheng, Lies Anne Severijnen, Tianna Zhao, Rob Willemsen, Marcel van der Weiden, Vincenzo Bonifati, Johan M. Kros |
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| Přispěvatelé: | Pathology, Clinical Genetics |
| Rok vydání: | 2013 |
| Předmět: |
Male
Pathology medicine.medical_specialty Cytoplasmic inclusion Neuropathology Biology Pathology and Forensic Medicine Progressive supranuclear palsy Cellular and Molecular Neuroscience Atrophy medicine Humans Aged Aged 80 and over Inclusion Bodies Synucleinopathies F-Box Proteins Putamen Brain Parkinson Disease General Medicine Human brain Middle Aged Multiple System Atrophy medicine.disease HEK293 Cells medicine.anatomical_structure Neurology alpha-Synuclein Female Neurology (clinical) Alzheimer's disease |
| Zdroj: | Journal of Neuropathology and Experimental Neurology, 72(6), 482-488. Oxford University Press |
| ISSN: | 0022-3069 |
| DOI: | 10.1097/nen.0b013e318293c586 |
| Popis: | Mutations in the gene encoding the F-box only protein 7 (FBXO7) cause PARK15, an autosomal recessive form of juvenile parkinsonism. Although the brain pathology in PARK15 patients remains unexplored, in vivo imaging displays severe loss of nigrostriatal dopaminergic terminals. Understanding the pathogenesis of PARK15 might therefore illuminate the mechanisms of the selective dopaminergic neuronal degeneration, which could also be important for understanding idiopathic Parkinson disease (PD). The expression of FBXO7 in the human brain remains poorly characterized, and its expression in idiopathic PD and different neurodegenerative diseases has not been investigated. Here, we studied FBXO7 protein expression in brain samples of normal controls (n = 9) and from patients with PD (n = 13), multiple system atrophy (MSA) (n = 5), Alzheimer disease (AD) (n = 5), and progressive supranuclear palsy (PSP) (n = 5) using immunohistochemistry with 2 anti-FBXO7 antibodies. We detected widespread brain FBXO7 immunoreactivity, with the highest levels in neurons of the cerebral cortex, putamen, and cerebellum. There were no major differences between normal and PD brains overall, but FBXO7 immunoreactivity was detected in large proportions of α-synuclein-positive inclusions (Lewy bodies, Lewy neurites, glial cytoplasmic inclusions), where it colocalized with α-synuclein in PD and MSA cases. By contrast, weak FBXO7 immunoreactivity was occasionally detected in tau-positive inclusions in AD and PSP. These findings suggest a role for FBXO7 in the pathogenesis of the synucleinopathies. Copyright |
| Databáze: | OpenAIRE |
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