Identification and cloning of highly epitopic regions of Clostridium novyi alpha toxin
| Autor: | Mohsen Mehrvarz, Najme Gord Noshahri, Ali Makhdoumi Kakhki, Behjat Majidi, Mohsen Fathi Najafi |
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| Rok vydání: | 2016 |
| Předmět: |
040301 veterinary sciences
Physiology medicine.disease_cause Microbiology Epitope law.invention 0403 veterinary science law Genetics medicine Homology modeling Molecular Biology biology Toxin Binding protein 0402 animal and dairy science 04 agricultural and veterinary sciences Cell Biology Clostridium novyi biology.organism_classification 040201 dairy & animal science Molecular biology Alpha toxin Clostridium novyi antigenic regions conformational epitope homology modeling Recombinant DNA Antitoxin General Agricultural and Biological Sciences Conformational epitope |
| Zdroj: | Volume: 40, Issue: 6 1219-1226 Turkish Journal of Biology |
| ISSN: | 1300-0152 1303-6092 |
| Popis: | The aim of the present study is to provide a strategy for predicting the production of antigenic regions of Clostridium novyi alpha toxin. The selection is based on B-cell epitopes and MHCII binding protein by immunoinformatics tools. The study resulted in identifying antigenic regions in the beginning and middle (3-17 and 965-997 amino acid residues) as suitable binders to MHCII and the carboxyl terminal of the protein (1800-1958 amino acid residues) in B-cell epitopes. The appropriate region in B-cell epitopes was chosen for cloning. The presence of recombinant protein was detected with immunological methods. Subsequently, BALB/C mice were immunized with the recombinant protein and alpha toxin, and the antibodies produced were evaluated by dot-blot and ELISA tests. After cloning the highly antigenic region (1799-1966 amino acid residues), the results of immunological tests showed that the recombinant protein reacts with antitoxin antibodies. Antirecombinant protein has a higher affinity to the alpha toxin than antialpha toxin. Thus, the carboxyl terminal of the protein (1799-1966 amino acid residues) in B-cell epitopes could be a proper candidate for a peptide vaccine against alpha toxin. |
| Databáze: | OpenAIRE |
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