Choline Transport Activity Regulates Phosphatidylcholine Synthesis through Choline Transporter Hnm1 Stability
| Autor: | Michael H. Ngo, Christopher R. McMaster, J. Pedro Fernández-Murray |
|---|---|
| Rok vydání: | 2013 |
| Předmět: |
Saccharomyces cerevisiae Proteins
Biological Transport Active Down-Regulation Saccharomyces cerevisiae Biochemistry Choline chemistry.chemical_compound Phosphatidylcholine Molecular Biology Endosomal Sorting Complexes Required for Transport biology Protein Stability Membrane transport protein Membrane Transport Proteins Ubiquitin-Protein Ligase Complexes Transporter Cell Biology Membrane transport Lipids Endocytosis Cell biology Transport protein Choline transporter Protein Transport chemistry Mutation Proteolysis Vacuoles Phosphatidylcholines biology.protein Choline transport |
| Zdroj: | Journal of Biological Chemistry. 288:36106-36115 |
| ISSN: | 0021-9258 |
| Popis: | Choline is a precursor for the synthesis of phosphatidylcholine through the CDP-choline pathway. Saccharomyces cerevisiae expresses a single high affinity choline transporter at the plasma membrane, encoded by the HNM1 gene. We show that exposing cells to increasing levels of choline results in two different regulatory mechanisms impacting Hnm1 activity. Initial exposure to choline results in a rapid decrease in Hnm1 mediated transport at the level of transporter activity, while chronic exposure results in Hnm1 degradation through an endocytic mechanism that depends on the ubiquitin ligase Rsp5 and the casein kinase 1 redundant pair Yck1/Yck2. We present details of how the choline transporter is a major regulator of phosphatidylcholine synthesis. |
| Databáze: | OpenAIRE |
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