Ligands of peroxisome proliferator-activated receptor-gamma increase the generation of vascular endothelial growth factor in vascular smooth muscle cells and in macrophages
| Autor: | Aldona Dembinska-Kiec, Alicja Jozkowicz, Ewa Piatkowska, Wojciech Placha, Jozef Dulak |
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| Rok vydání: | 2002 |
| Předmět: |
Lipopolysaccharides
Transcriptional Activation Vascular Endothelial Growth Factor A medicine.medical_specialty Vascular smooth muscle Angiogenesis Peroxisome proliferator-activated receptor Receptors Cytoplasmic and Nuclear Antineoplastic Agents Endothelial Growth Factors Ligands Transfection General Biochemistry Genetics and Molecular Biology Cell Line chemistry.chemical_compound Mice Downregulation and upregulation Ciglitazone Internal medicine medicine Myocyte Animals Hypoglycemic Agents RNA Messenger Promoter Regions Genetic Transcription factor Cells Cultured chemistry.chemical_classification Lymphokines Dose-Response Relationship Drug Prostaglandin D2 Reverse Transcriptase Polymerase Chain Reaction Vascular Endothelial Growth Factors Macrophages Muscle Smooth Cell biology Rats Up-Regulation Vascular endothelial growth factor Thiazoles Endocrinology chemistry Thiazolidinediones Endothelium Vascular Interleukin-1 Transcription Factors |
| Zdroj: | Acta biochimica Polonica. 47(4) |
| ISSN: | 0001-527X |
| Popis: | Peroxisome proliferator-activated receptors-gamma (PPARgamma) are ligand-inducible transcription factors of the nuclear hormone receptor superfamily. We examined the effect of PPARgamma activation on the generation of vascular endothelial growth factor (VEGF), one of the major angiogenic agents. Rat vascular smooth muscle cells (VSMC) and murine macrophages RAW264.7 were incubated for 24 h with PPARgamma activators: prostaglandin J2 and ciglitazone. PPARgamma were expressed in VSMC and RAW cells and their activity was upregulated in the presence of PGJ2 and ciglitazone. Incubation of the cells with PPARgamma activators significantly augmented the release of VEGF protein into the media, both in resting and in IL-1beta- or LPS-stimulated cultures. The higher protein generation was connected with the increased expression of mRNA and transcriptional activation of VEGF promoter. We conclude that the activation of PPARgamma upregulates the generation of VEGF and may be involved in the regulation of angiogenesis. |
| Databáze: | OpenAIRE |
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