Cinobufagin enhances the protective efficacy of formalin-inactivated Salmonella typhimurium vaccine through Th1 immune response
| Autor: | Hai-Qing Shen, Xu-Bin Wei, Dao-Xiu Xu, Shuai-Cheng Wu, Peng-Fei Yi, Li-Yan Zhang, Yun-Xing Fu, Zhen-Qiang Cui, Xun Guo, Ben-Dong Fu |
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| Rok vydání: | 2016 |
| Předmět: |
Salmonella typhimurium
Serum 0301 basic medicine Salmonella Lipopolysaccharide Salmonella Vaccines medicine.medical_treatment 030106 microbiology Spleen medicine.disease_cause Microbiology Fixatives 03 medical and health sciences chemistry.chemical_compound Adjuvants Immunologic In vivo Formaldehyde medicine Splenocyte Animals Cinobufagin Immunization Schedule Cell Proliferation Mice Inbred ICR Salmonella Infections Animal biology Th1 Cells Antibodies Bacterial Survival Analysis Bufanolides Disease Models Animal 030104 developmental biology Infectious Diseases medicine.anatomical_structure Cytokine Vaccines Inactivated chemistry Concanavalin A Immunoglobulin G Leukocytes Mononuclear biology.protein Alum Compounds Cytokines |
| Zdroj: | Microbial Pathogenesis. 99:264-270 |
| ISSN: | 0882-4010 |
| DOI: | 10.1016/j.micpath.2016.08.036 |
| Popis: | Cinobufagin (CBG), one active ingredient isolated from Venenum Bufonis, has been demonstrated to have immunoregulatory effect. The aim of this study was to investigate whether CBG can enhance the protective efficacy of formalin-inactivated Salmonella typhimurium (FIST) in mice. ICR mice were immunized with FIST (106 CFU/mouse) alone or mixed with CBG (10, 20, and 40 μg) or alum (200 μg) on day 1 and day 15. Two weeks after the second immunization, serum and spleen were sampled for measuring FIST-specific antibody levels, cytokine levels, and splenocyte proliferation. The results showed that CBG enhanced FIST-specific IgG and IgG2a, the levels of interferon-gamma (IFNγ) and nitric oxide (NO), and the splenocyte proliferation response induced by concanavalin A, lipopolysaccharide, and FIST. In vivo protection studies showed that CBG significantly decreased the bacterial burdens in the spleen and prolonged the survival time of FIST-immunized mice challenged with live Salmonella typhimurium. In vivo IFNγ neutralization led to a significant reduction in FIST-specific IgG2a and IFNγ levels, and in the protective efficacy in CBG/FIST-immunized mice. In conclusion, CBG enhances the protective efficacy of formalin-inactivated Salmonella typhimurium vaccine by promoting the Th1 immune response. |
| Databáze: | OpenAIRE |
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