Alterations in the gene expression of drug and arachidonic acid-metabolizing Cyp450 in the livers of controlled and uncontrolled insulin-dependent diabetic mice
| Autor: | Abdulhasib Kilani, Yazun Jarrar, Mariam Hasan, Lauy Al-Essa, Walid Al-Qerem |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
insulin medicine.medical_specialty UGTs medicine.medical_treatment CYP450s 03 medical and health sciences chemistry.chemical_compound Internal medicine Diabetes mellitus Gene expression arachidonic acid Internal Medicine medicine Targets and Therapy [Diabetes Metabolic Syndrome and Obesity] Original Research Pharmacology diabetes Fatty acid metabolism business.industry Insulin CYP1A2 medicine.disease drug metabolism Streptozocin 030104 developmental biology Endocrinology chemistry gene expression Arachidonic acid business Drug metabolism |
| Zdroj: | Diabetes, Metabolic Syndrome and Obesity: Targets and Therapy |
| ISSN: | 1178-7007 |
| Popis: | Yazun Bashir Jarrar, Luay Al-Essa, Abdulhasib Kilani, Mariam Hasan, Walid Al-Qerem Department of Pharmacy, College of Pharmacy, Al-Zaytoonah University of Jordan, Airport street, Amman, Jordan Background: Diabetic patients have lower capacity to metabolize drugs in comparison to normal people. Therefore, the present study aimed to investigate the alterations in gene expression of drug and arachidonic acid metabolizing cytochrome p450s (cyp450s) in the livers of controlled (CDM) and uncontrolled (UDM) insulin-dependent diabetic mice. Methods: Balb/c mice were treated with single dose of streptozocin (240 mg/kg) to induce diabetes and compared with control group, which was treated with citric buffer (pH =4.5). After 3 days, the blood glucose level was measured to confirm the induction of diabetes. Normalization of blood glucose level in diabetic mice was achieved after 0.1 mL/kg Mixtard® insulin therapy for more 5 days. Then, the mice livers were isolated to extract RNA and convert it to cDNA. The gene expression of 14 genes, which play a major role in drug and arachidonic acid metabolism, were measured using quantitative real-time polymerase chain reaction technique. Results: It was found that the gene expression was downregulated (ANOVA test, P-value |
| Databáze: | OpenAIRE |
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