Observations on the growth and plaque assay of BK virus in cultured human and monkey cells
| Autor: | John S. Colter, Jutta Seehafer, Pat Carpenter, Donald N. Downer |
|---|---|
| Rok vydání: | 1978 |
| Předmět: |
viruses
Viral Plaque Assay Biology medicine.disease_cause Kidney Virus Replication Virus Virology medicine Animals Humans Lung Virus quantification urogenital system Muscles HEK 293 cells Embryo Haplorhini Embryo Mammalian Molecular biology BK virus Titer medicine.anatomical_structure BK Virus Vero cell Polyomavirus |
| Zdroj: | The Journal of general virology. 38(2) |
| ISSN: | 0022-1317 |
| Popis: | Summary Although human embryo kidney (HEK), muscle (HEM) and lung (HEL) cells are capable of supporting the replication of BK virus (BKV) through passage levels 9, 12 and 12 respectively, only third, fourth and fifth passage level HEK cells were found to be satisfactory for the plaque assay of the virus. BSC-1 and VERO cells can also be used for the plaque assay of BKV. However, in HEK cells plaques can be visualized in 20 days (compared to 28 days in BSC-1 cells), and since in VERO cells the plaques are poorly defined and the titre about 1 log10 lower than in either HEK or BSC-1 cells, HEK cells were the ones chosen for use. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|