A rhamnose-binding lectin from Rhodnius prolixus and the impact of its silencing on gut bacterial microbiota and Trypanosoma cruzi
Autor: | A.L. Dos Santos, Norman A. Ratcliffe, Cicero B. Mello, João Pacheco, Paul Dyson, Reinaldo B Geraldo, A. Sibajev, Victor G O Evangelho, C.A.C. Araújo, P.J. Waniek, Patrícia Azambuja, Helena Carla Castro |
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Rok vydání: | 2021 |
Předmět: |
Fish Proteins
0301 basic medicine Rhamnose Trypanosoma cruzi 030231 tropical medicine Immunology Disease Vectors Microbiology Defensins 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Lectins RNA Ribosomal 16S Gene expression Animals Chagas Disease Gene Silencing Rhodnius prolixus Defensin biology Lectin Rhamnose binding biology.organism_classification Immunity Innate Gastrointestinal Microbiome Molecular Docking Simulation RNA silencing 030104 developmental biology chemistry Structural Homology Protein Rhodnius biology.protein Insect Proteins Developmental Biology |
Zdroj: | Developmental & Comparative Immunology. 114:103823 |
ISSN: | 0145-305X |
Popis: | Lectins are ubiquitous proteins involved in the immune defenses of different organisms and mainly responsible for non-self-recognition and agglutination reactions. This work describes molecular and biological characterization of a rhamnose-binding lectin (RBL) from Rhodnius prolixus, which possesses a 21 amino acid signal peptide and a mature protein of 34.6 kDa. The in-silico analysis of the primary and secondary structures of RpLec revealed a lectin domain fully conserved among previous insects studied. The three-dimensional homology model of RpLec was similar to other RBL-lectins. Docking predictions with the monosaccharides showed rhamnose and galactose-binding sites comparable to Latrophilin-1 and N-Acetylgalactosamine-binding in a different site. The effects of RpLec gene silencing on levels of infecting Trypanosoma cruzi Dm 28c and intestinal bacterial populations in the R. prolixus midgut were studied by injecting RpLec dsRNA into the R. prolixus hemocoel. Whereas T. cruzi numbers remained unchanged compared with the controls, numbers of bacteria increased significantly. The silencing also induced the up regulation of the R. prolixus defC (defensin) expression gene. These results with RpLec reveal the potential importance of this little studied molecule in the insect vector immune response and homeostasis of the gut bacterial microbiota. |
Databáze: | OpenAIRE |
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