Exposure to Bacterial CpG DNA Protects from Airway Allergic Inflammation by Expanding Regulatory Lung Interstitial Macrophages
| Autor: | Dimitri Pirottin, Sabine Olivier, Florent Ginhoux, Laurent Gillet, Fabrice Bureau, Cláudia A Fernandes, Geneviève Paulissen, Catherine Sabatel, Didier Cataldo, Coraline Radermecker, Thomas Marichal, Pascale Quatresooz, Svetoslav Chakarov, Xue Xiao, Christophe Desmet, Jean-Claude Sirard, Laurence Fievez, Marie Toussaint, Hicham Bouabe |
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| Přispěvatelé: | Sirard, Jean-Claude, Groupe Interdisciplinaire de Génoprotéomique Appliquée (GIGA-Research), Université de Liège, Faculté de Médecine Vétérinaire [Liège], Singapore Immunology Network (SIgN), Biomedical Sciences Institute (BMSI), National Heart and Lung Institute [London, UK] (NHLI), Imperial College London, Fundamental and Applied Research for Animals & Health (FARAH), Centre de recherche sur les protéines prions [Liège, Belgique] (CRPP), Centre d’Infection et d’Immunité de Lille - INSERM U 1019 - UMR 9017 - UMR 8204 (CIIL), Institut Pasteur de Lille, Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Lille-Centre Hospitalier Régional Universitaire [Lille] (CHRU Lille)-Centre National de la Recherche Scientifique (CNRS), The Babraham Institute [Cambridge, UK], Walloon Excellence in Life sciences and BIOtechnology [Liège] (WELBIO), The Laboratory of Cellular and Molecular Immunology is supported by the F.R.S.-FNRS for the FRFS-WELBIO under grant CR-2012S-01R, by the Belgian Program on Interuniversity Attraction Poles (IUAP, T-TIME, P7/39), and by an 'Action de Recherche Concertée de la Fédération Wallonie-Bruxelles de Belgique' (12/07-03-ITPKC). C.S. and C.R. are research fellows of the F.R.S.-FNRS, L.F. is supported by the FRFS-WELBIO (CR-2012S-01R), T.M. is supported by the Acteria Foundation, and C.D. and T.M. are research associates of the F.R.S.-FNRS., We thank Dr. François Trottein (Institut Pasteur de Lille) for providing Influenza A virus, Prof. Hans Nauwynck (Ghent University) for advice, Dr. Natalia Kosovilka from the Stanford PAN facility, Dr. Sandra Ormenese and Raafat Stephan from the Cell Imaging and Flow Cytometry GIGA Platform, Dr. Pierre Drion and other staff members from the Mouse Facility and Transgenics GIGA Platform, Dr. Chantal Humblet from the GIGA Immunohistology Platform, and Raja Fares, Cédric François, and Ilham Sbai for excellent technical and secretarial assistance., Centre National de la Recherche Scientifique (CNRS)-Centre Hospitalier Régional Universitaire [Lille] (CHRU Lille)-Université de Lille-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut Pasteur de Lille, Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP) |
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
CCR2 [SDV]Life Sciences [q-bio] MESH: Flow Cytometry MESH: Mice Knockout Regulatory macrophages hygiene hypothesis Allergic sensitization Mice TLR9 0302 clinical medicine Immunology and Allergy MESH: Animals Mice Knockout MESH: Macrophages/immunology Flow Cytometry 3. Good health [SDV] Life Sciences [q-bio] MESH: Spleen/immunology Chemotaxis Leukocyte Interleukin 10 Infectious Diseases medicine.anatomical_structure Oligodeoxyribonucleotides CpG site 030220 oncology & carcinogenesis IL-10 monocytes MESH: Respiratory Hypersensitivity/immunology DNA Bacterial MESH: Macrophages Alveolar/immunology Immunology Biology Allergic inflammation lung 03 medical and health sciences MESH: Oligodeoxyribonucleotides/immunology MESH: Mice Inbred C57BL CpG regulatory macrophages Macrophages Alveolar MESH: Macrophage Activation/immunology Hypersensitivity Respiratory Hypersensitivity medicine Animals MESH: Mice Macrophages Monocyte MESH: DNA Bacterial/immunology Macrophage Activation asthma MESH: Chemotaxis Leukocyte/immunology respiratory tract diseases Mice Inbred C57BL Disease Models Animal 030104 developmental biology spleen MESH: Hypersensitivity/immunology MESH: Disease Models Animal |
| Zdroj: | Immunity Immunity, 2017, 46 (3), pp.457-473. ⟨10.1016/j.immuni.2017.02.016⟩ Immunity, Elsevier, 2017, 46 (3), pp.457-473. ⟨10.1016/j.immuni.2017.02.016⟩ |
| ISSN: | 1074-7613 |
| DOI: | 10.1016/j.immuni.2017.02.016 |
| Popis: | International audience; Living in a microbe-rich environment reduces the risk of developing asthma. Exposure of humans or mice to unmethylated CpG DNA (CpG) from bacteria reproduces these protective effects, suggesting a major contribution of CpG to microbe-induced asthma resistance. However, how CpG confers protection remains elusive. We found that exposure to CpG expanded regulatory lung interstitial macrophages (IMs) from monocytes infiltrating the lung or mobilized from the spleen. Trafficking of IM precursors to the lung was independent of CCR2, a chemokine receptor required for monocyte mobilization from the bone marrow. Using a mouse model of allergic airway inflammation, we found that adoptive transfer of IMs isolated from CpG-treated mice recapitulated the protective effects of CpG when administered before allergen sensitization or challenge. IM-mediated protection was dependent on IL-10, given that Il10(-/-) CpG-induced IMs lacked regulatory effects. Thus, the expansion of regulatory lung IMs upon exposure to CpG might underlie the reduced risk of asthma development associated with a microbe-rich environment. |
| Databáze: | OpenAIRE |
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