Mixed-Culture Interactions I. Commensalism of Proteus vulgaris with Saccharomyces cerevisiae in Continuous Culture
| Autor: | Kathleen Culbert, Henry R. Bungay, Adnan Shindala, Noel R. Krieg |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 1965 |
| Předmět: |
Niacinamide
Population Proteus vulgaris Saccharomyces cerevisiae Microbiology Saccharomyces Niacin education Symbiosis Molecular Biology education.field_of_study Bacteriological Techniques biology Bacteria Research Nicotinic Acids Taxonomy Ecology Morphology and Structure and Microbiological Methods biology.organism_classification Proteus Yeast Culture Media Chemically defined medium |
| Popis: | Shindala, Adnan (Virginia Polytechnic Institute, Blacksburg), Henry R. Bungay III, Noel R. Krieg, and Kathleen Culbert . Mixed-culture interactions. I. Commensalism of Proteus vulgaris with Saccharomyces cerevisiae in continuous culture. J. Bacteriol. 89: 693–696. 1965.—A chemically defined medium was selected which supported the growth of Saccharomyces cerevisiae , but not Proteus vulgaris , in pure culture; however, P. vulgaris grew in mixed culture with the yeast. Steady-state populations of each organism in mixed culture at various dilution rates were enumerated with a Coulter electronic counter. The size differences in the organisms permitted easy resolution. An essential niacinlike factor elaborated by the yeast and required by the bacterium caused a dependence of the bacterium on the growth of the yeast. At high dilution rates causing wash-out, the bacterial population continued to reflect changes in the numbers of yeast. The numbers of S. cerevisiae were identical in pure culture or in mixed culture; thus, the interaction is a true commensalism. A single addition of niacin or related compound was made to a steady-state mixed culture, and the dependence of the bacterium on the yeast was upset. The numbers of the bacteria rose, causing a decrease in the yeast population, until continued pumping diluted the added niacin and restored the initial steady state. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|