DEP-1 protein tyrosine phosphatase inhibits proliferation and migration of colon carcinoma cells and is upregulated by protective nutrients
| Autor: | K K Balavenkatraman, Frank-D. Böhmer, Beatrice L. Pool-Zobel, T Kautenburger, Karlheinz Friedrich, E Jandt, Arne Östman |
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| Rok vydání: | 2006 |
| Předmět: |
Cancer Research
Transcription Genetic Protein tyrosine phosphatase medicine.disease_cause Cell Movement Protein Phosphatase 1 RNA Neoplasm RNA Small Interfering Cells Cultured Receptor-Like Protein Tyrosine Phosphatases Class 3 Cell migration respiratory system Cell biology Neoplasm Proteins Up-Regulation Butyrates Biochemistry Enzyme Induction Malus Colonic Neoplasms RNA Interference Chemokines CXC Cell Division Adenoma Colon Recombinant Fusion Proteins Phosphatase Down-Regulation Butyrate Biology Adenocarcinoma Transfection complex mixtures Phenols Cell Line Tumor Genetics medicine Anticarcinogenic Agents Humans RNA Messenger Molecular Biology Flavonoids Tea Cell growth Plant Extracts Polyphenols Protein phosphatase 1 Epithelial Cells Chemokine CXCL12 respiratory tract diseases Cell culture Lysophospholipids Protein Tyrosine Phosphatases Carcinogenesis |
| Zdroj: | Oncogene. 25(47) |
| ISSN: | 0950-9232 |
| Popis: | The transmembrane protein-tyrosine phosphatase (PTP) DEP-1 (density-enhanced phosphatase) is a candidate tumor suppressor in the colon epithelium. We have explored the function of DEP-1 in colon epithelial cells by inducible re-expression in a DEP-1-deficient human colon cancer cell line. Density-enhanced phosphatase-1 re-expression led to profound inhibition of cell proliferation and cell migration, and was associated with cytoskeletal rearrangements. These effects were dependent on the PTP activity of DEP-1 as they were not observed with cells expressing the catalytically inactive DEP-1 C1239S variant. shRNA-mediated suppression of DEP-1 in a colon epithelial cell line with high endogenous DEP-1 levels enhanced proliferation, further supporting the antiproliferative function of DEP-1. Nutrients, which are considered to be chemoprotective with respect to colon cancer development, including butyrate, green tea and apple polyphenols, had the capacity to elevate transcription of endogenous DEP-1 mRNA and expression of DEP-1 protein. Upregulation of DEP-1 expression, and in turn inhibition of cell growth and migration may present a previously unrecognized mechanism of chemoprevention by nutrients. |
| Databáze: | OpenAIRE |
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