Nucleotide sequence of the Kaposi sarcoma-associated herpesvirus (HHV8)
| Autor: | James J. Russo, Yuan Chang, Isidore S. Edelman, Chen J, Minchen Chien, Patrick S. Moore, Roy A. Bohenzky, Peruzzi D, John Preston Parry, Maddalena D, Yan M |
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| Rok vydání: | 1996 |
| Předmět: |
Genetics
Multidisciplinary Base Sequence Genes Viral viruses Molecular Sequence Data Nucleic acid sequence Chromosome Mapping Genome Viral Biological Sciences Biology medicine.disease_cause biology.organism_classification Molecular biology Herpesvirus 8 Human medicine Cosmid Humans Rhadinovirus Coding region Genomic library Kaposi's sarcoma-associated herpesvirus ORFS Sarcoma Kaposi Gene |
| Zdroj: | Proceedings of the National Academy of Sciences. 93:14862-14867 |
| ISSN: | 1091-6490 0027-8424 |
| DOI: | 10.1073/pnas.93.25.14862 |
| Popis: | The genome of the Kaposi sarcoma-associated herpesvirus (KSHV or HHV8) was mapped with cosmid and phage genomic libraries from the BC-1 cell line. Its nucleotide sequence was determined except for a 3-kb region at the right end of the genome that was refractory to cloning. The BC-1 KSHV genome consists of a 140.5-kb-long unique coding region flanked by multiple G+C-rich 801-bp terminal repeat sequences. A genomic duplication that apparently arose in the parental tumor is present in this cell culture-derived strain. At least 81 ORFs, including 66 with homology to herpesvirus saimiri ORFs, and 5 internal repeat regions are present in the long unique region. The virus encodes homologs to complement-binding proteins, three cytokines (two macrophage inflammatory proteins and interleukin 6), dihydrofolate reductase,bcl-2, interferon regulatory factors, interleukin 8 receptor, neural cell adhesion molecule-like adhesin, and a D-type cyclin, as well as viral structural and metabolic proteins. Terminal repeat analysis of virus DNA from a KS lesion suggests a monoclonal expansion of KSHV in the KS tumor. |
| Databáze: | OpenAIRE |
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