The D-type alfalfa cyclin gene cycMs4 complements G1 cyclin-deficient yeast and is induced in the G1 phase of the cell cycle

Autor: Dang Thi Cam Ha, Marlis Dahl, Erwin Heberle-Bors, Rainer Hubmann, Heribert Hirt, Ines Swoboda, László Bögre, Irute Meskiene
Jazyk: angličtina
Rok vydání: 1995
Předmět:
Cyclin D
Meristem
Molecular Sequence Data
Cyclin A
Cyclin B
Gene Expression
Saccharomyces cerevisiae
Plant Science
Genes
Plant
Plant/isolation & purification/metabolism Recombinant Proteins/biosynthesis Saccharomyces cerevisiae/cytology/genetics/growth & development
Cyclin Gene
Cyclin-dependent kinase
Cyclins
Amino Acid Sequence Base Sequence Cell Cycle/*physiology Cyclins/*biosynthesis/genetics G1 Phase *Gene Expression *Genes
Amino Acid Sequence
Plant Proteins
Base Sequence
biology
Plant Genetic Complementation Test Medicago sativa/cytology/*genetics/metabolism Meristem Molecular Sequence Data *Plant Proteins RNA
Cell Cycle
Genetic Complementation Test
G1 Phase
Cell Biology
Faculty of Science\Biological Science
Cell cycle
Recombinant Proteins
Cell biology
RNA
Plant
biology.protein
Cyclin-dependent kinase 6
Cyclin A2
Research Article
Medicago sativa
Zdroj: Plant Cell, 7 (11)
Popis: Cyclins are key regulators of the cell cycle in all eukaryotes. In alfalfa, we have previously isolated three B-type cyclins. The closely related cycMs1 and cycMs2 genes are expressed primarily during the G2 and M phases and are most likely mitotic cyclins; expression of the cycMs3 gene is induced in the G0-to-G1 transition, when cells reenter the cell cycle. By complementation of G1 cyclin-deficient yeast cells, a novel alfalfa cyclin, designated cycMs4, was isolated. The predicted amino acid sequence of the cycMs4 gene is most similar to that of the Arabidopsis cyclin delta 3 gene. CycMs4 and cyclin delta 3 belong to the class of D-type cyclins and contain PEST-rich regions and a retinoblastoma binding motif. When comparing expression levels in different organs, cycMs4 transcripts were present predominantly in roots. Whereas expression of the cycMs4 gene was cell cycle-regulated in suspension-cultured cells, transcription in roots was observed to depend also on the positional context of the cell. When differentiated G0-arrested leaf cells were induced to resume cell division by treatment with plant hormones, cycMs4 transcription was induced before the onset of DNA synthesis. Whereas this induction was preceded by that of the cycMs3 gene, cycMs2 expression occurred later and at the same time as mitotic activity. These data suggest that cycMs4 plays a role in the G1-to-S transition and provide a model to investigate the plant cell cycle at the molecular level.
Databáze: OpenAIRE
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