Hepatotoxicity of nutmeg: A pilot study based on metabolomics
| Autor: | Huiya Yuan, Lina Gao, Dawei Guan, Zhipeng Cao, Wei Xia, Xiaoyu Zhang |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Male Time Factors medicine.medical_treatment H&E stain Poison control Pilot Projects RM1-950 Biology Pharmacology Abuse Liver function Myristica 03 medical and health sciences Mice 0302 clinical medicine Liver Function Tests Nutmeg medicine Animals Metabolomics KEGG Saline Dose-Response Relationship Drug Plant Extracts Hepatotoxicity General Medicine biology.organism_classification 030104 developmental biology medicine.anatomical_structure 030220 oncology & carcinogenesis Hepatocyte Seeds Hepatocytes Myristica fragrans Therapeutics. Pharmacology Chemical and Drug Induced Liver Injury |
| Zdroj: | Biomedicine & Pharmacotherapy, Vol 131, Iss, Pp 110780-(2020) |
| ISSN: | 0753-3322 |
| Popis: | Incidences of abuse and poisoning have been reported for nutmeg, a household spice made from grinding the seed of Myristica fragrans, owing to its hallucinogenic properties. However, there have been no reports on nutmeg hepatotoxicity in relation to dose and duration of exposure. To investigate the hepatotoxicity of different nutmeg exposure durations and doses, male mice were administered daily with normal saline, 1.0 g/kg nutmeg, or 4.0 g/kg nutmeg by intragastrical gavage for either 7 or 14 days (for a total of six treatment groups, n = 6). Body weight of each mouse was monitored daily. Histological analysis of liver tissues was performed using hematoxylin and eosin (H&E) staining to investigate the morphological changes in hepatocytes. Serum aspartate aminotransferase (AST) and alanine aminotransferase (ALT) levels were determined using enzyme-linked immunosorbent assay (ELISA) to investigate liver function. Metabolomics and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analyses were performed between treatment groups for identifying differential metabolites. Mice in the nutmeg exposure groups exhibited slow growth trends, hepatocyte damage, and significantly elevated serum AST and ALT levels associated with nutmeg dose and exposure duration. Metabolomics and KEGG enrichment pathway analyses also revealed differential levels of some metabolites related to liver function upon nutmeg exposure. Therefore, the present study reasonably speculates that nutmeg exposure may cause liver damage and affect liver function depending on the dose and duration. |
| Databáze: | OpenAIRE |
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