Electroconvulsive stimulation transiently enhances the permeability of the rat blood-brain barrier and induces astrocytic changes
| Autor: | Declan M. McLoughlin, Masanobu Ito, Toshihito Suzuki, Heii Arai, Tomoya Kinjo, Koichiro Ichimura, Akiko Furuta, Kuerban Bolati |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Stimulation S100 Calcium Binding Protein beta Subunit Motor Activity Pharmacology Inferior vena cava Capillary Permeability Random Allocation 03 medical and health sciences 0302 clinical medicine Seizures In vivo fluorescence medicine Animals Rats Wistar Fluorescent Dyes Electroshock Tight junction Chemistry General Neuroscience Optical Imaging Rat blood Microscopy Electron Spectrometry Fluorescence 030104 developmental biology medicine.anatomical_structure medicine.vein Blood-Brain Barrier Permeability (electromagnetism) Astrocytes cardiovascular system Female Fluorescein Sodium fluorescein Neuroscience 030217 neurology & neurosurgery Astrocyte |
| Zdroj: | Brain Research Bulletin. 128:92-97 |
| ISSN: | 0361-9230 |
| DOI: | 10.1016/j.brainresbull.2016.11.011 |
| Popis: | The blood-brain barrier (BBB) plays important roles in both the physiological and pharmacological state of the brain. Transiently enhancing the permeability of the BBB may allow use of more types of medications for neuropsychiatric diseases. Several studies have demonstrated that seizures cause a transient decrease in BBB integrity. We studied the timing of BBB changes following seizures and the role of astrocytes in this process. Rats received 10 applications of electroconvulsive stimulation (ECS). They were then infused with sodium fluorescein, a fluorescent substance that rarely passes the BBB, via the inferior vena cava. After 120 min of circulation, the amount of sodium fluorescein in the brain was measured by two methods in vivo fluorescence imaging (total radiant efficiency) and the brain concentration of sodium fluorescein. To assess any changes to the BBB, we measured S100Β in serum, which is a standard marker of BBB breakdown that is expressed by astrocytes. We also examined ultrastructural changes following ECS. Total radiant efficiency and the brain concentration of sodium fluorescein were significantly increased in treated rats compared to controls when sodium fluorescein was injected immediately after ECS but not when the injection was performed more than 15 min after ECS. Astrocytic endfeet showed swelling around brain capillaries following ECS. In conclusion, ECS transiently enhances the permeability of the BBB, which may be accompanied by changes in astrocytic endfeet. |
| Databáze: | OpenAIRE |
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