Hepatitis B Virus E Antigen (Hbeag) May Have A Negative Effect On Dendritic Cell Generation
| Autor: | Aynur Başalp, Ibrahim Hatipoglu, Duygu Ercan, Ahmet Tarik Baykal, Ceyda Acilan, Deniz Durali |
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| Rok vydání: | 2014 |
| Předmět: |
Proteomics
Hepatitis B virus Proteome Cell Survival Immunology Cell Count Dendritic Cell HBeAg medicine.disease_cause Virus Mice Immune system Antigen medicine Animals Antigens Ly Humans Immunology and Allergy Myeloid Cells Hepatitis B e Antigens LC-MS/MS business.industry virus diseases Cell Differentiation Dendritic Cells Hematology Dendritic cell Virology digestive system diseases CD11c Antigen Chronic infection medicine.anatomical_structure Bone marrow business |
| Popis: | WOS: 000345541900006 PubMed ID: 25150150 Hepatitis B virus (HBV) continues to be a serious worldwide health problem despite the use of protective HBV vaccines and therapeutic regimens against chronic HBV infection. Chronic HBV patients cannot induce sufficient immune responses against the virus. HBV and its antigens are believed to suppress immune responses during chronic infection. Hence, studying the role of HBV in immune suppression is very important for the development of alternative therapeutic strategies for HBV infections. In the present study, we investigated the effect of Hepatitis B virus e antigen (HBeAg) on the generation of bone marrow derived dendritic cells (BMDCs) and the stimulation of plasmacytoid DCs (pDCs). In the presence of HBeAg, the ratio of BMDCs was decreased, but the ratio of CD11b(+)Ly6G(+) immature myeloid cells was increased. The expression of 47 proteins was also changed during HBeAg treatment; however, CpG-induced MHC-II expression on pDCs was not affected. Our results indicate that HBeAg may have a negative effect on the generation of DCs from bone morrow precursors. (C) 2014 Elsevier GmbH. All rights reserved. Scientific and Technological Research Council of Turkey (TUBITAK) [108S300, 110S497, 105G0656] This study was supported by grants nos. 108S300, 110S497 and 105G0656 from The Scientific and Technological Research Council of Turkey (TUBITAK). We thank Dr. Betul Guloglu for critically reviewing the manuscript and Duygu OZCELIK for her excellent technical assistance. |
| Databáze: | OpenAIRE |
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