Streamlined process for the chemical synthesis of RNA using 2'-O-thionocarbamate-protected nucleoside phosphoramidites in the solid phase
| Autor: | Fernando Ferreira, Douglas J. Dellinger, John O. Turner, Zoltan Timar, Marvin H. Caruthers, Jeffrey R. Sampson, Joel Myerson, Kenneth W. Hill, Zoltán Kupihár, Agnieszka B. Sierzchala, James A. Powell, Geraldine F Dellinger |
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| Rok vydání: | 2011 |
| Předmět: |
Phosphoramidite
Molecular Structure Sulfur Compounds Morpholines RNA Improved method Nucleosides General Chemistry Biochemistry Combinatorial chemistry Chemical synthesis Catalysis chemistry.chemical_compound Colloid and Surface Chemistry Monomer Organophosphorus Compounds chemistry Scientific method Phase (matter) Nucleoside |
| Zdroj: | Journal of the American Chemical Society. 133(30) |
| ISSN: | 1520-5126 |
| Popis: | An improved method for the chemical synthesis of RNA was developed utilizing a streamlined method for the preparation of phosphoramidite monomers and a single-step deprotection of the resulting oligoribonucleotide product using 1,2-diamines under anhydrous conditions. The process is compatible with most standard heterobase protection and employs a 2'-O-(1,1-dioxo-1λ(6)-thiomorpholine-4-carbothioate) as a unique 2'-hydroxyl protective group. Using this approach, it was demonstrated that the chemical synthesis of RNA can be as simple and robust as the chemical synthesis of DNA. |
| Databáze: | OpenAIRE |
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