Expression profiling of ETO2-regulated miRNAs in erythroid cells: Possible influence on miRNA abundance
| Autor: | Yoko Okitsu, Noriko Fukuhara, Kenichi Ishizawa, Hideo Harigae, Yasushi Onishi, Tohru Fujiwara, Yuna Katsuoka |
|---|---|
| Rok vydání: | 2013 |
| Předmět: |
CBF1
core-binding factor 1 Genetics LMO2 Small interfering RNA biology SCF stem cell factor ETO2 IL-3 interleukin 3 Article General Biochemistry Genetics and Molecular Biology IMDM Iscove’s Modified Dulbecco’s Media LMO2 LIM domain only 2 Gene expression profiling cDNA complementary DNA ETO2 (CBFA2T3) core-binding factor runt domain alpha subunit 2 translocated to 3 Histone siRNA small interfering RNA microRNA biology.protein Gene silencing Erythropoiesis RPMI Roswell Park Memorial Institute Gene miRNA TAL1 |
| Zdroj: | FEBS Open Bio |
| ISSN: | 2211-5463 |
| DOI: | 10.1016/j.fob.2013.10.004 |
| Popis: | ETO2 is a component of a protein complex containing master regulators of hematopoiesis, including GATA-1 and SCL/TAL1, and also has RNA binding properties. Although ETO2 has been reported to repress GATA-1 target genes through histone deacetylation of the target gene loci in erythroid cells, little is known about the contribution of ETO2 to microRNA (miRNA) regulation. Here, we conducted miRNA profiling in ETO2-overexpressing and ETO2-silenced K562 cells. The analysis suggests that ETO2 positively regulates the abundance of mature miRNAs, including miR-21, miR-29b and let-7e. Our data suggest a novel mode of ETO2-mediated target gene repression via effects on miRNA expression. Highlights • miRNA profiling was conducted in ETO2-overexpressing and ETO2-silenced K562 cells. • ETO2 positively regulates the abundance of miRNA. • ETO2 positively regulates the expression of miR-21, miR-29b and let-7e. |
| Databáze: | OpenAIRE |
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