Mannosylated Nanoparticles for Oral Immunotherapy in a Murine Model of Peanut Allergy
| Autor: | Carlos Gamazo, Juan M. Irache, Iván Peñuelas, Nekane Martín-Arbella, Ana Brotons-Canto, Mark Gumbleton, Gemma Quincoces, Muthanna Abdulkarim |
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| Rok vydání: | 2019 |
| Předmět: |
Male
Allergy Arachis Polymers medicine.medical_treatment Peanut allergy Administration Oral Pharmaceutical Science 02 engineering and technology Pharmacology 030226 pharmacology & pharmacy Immunoadjuvant Mice 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Adjuvants Immunologic medicine Animals Immunologic Factors Peanut Hypersensitivity Rats Wistar food and beverages Mannosamine Immunotherapy 021001 nanoscience & nanotechnology medicine.disease Intestinal epithelium Rats Disease Models Animal chemistry Nanoparticles Female 0210 nano-technology Anaphylaxis Conjugate |
| Zdroj: | Journal of Pharmaceutical Sciences. 108:2421-2429 |
| ISSN: | 0022-3549 |
| Popis: | Peanut allergy is one of the most prevalent and severe of food allergies with no available cure. The aim of this work was to evaluate the potential of an oral immunotherapy based on the use of a roasted peanut extract encapsulated in nanoparticles with immunoadjuvant properties. For this, a polymer conjugate formed by the covalent binding of mannosamine to the copolymer of methyl vinyl ether and maleic anhydride was first synthetized and characterized. Then, the conjugate was used to prepare nanoparticles with an important capability to diffuse through the mucus layer and reach, in a large extent, the intestinal epithelium, including Peyer's patches. Their immunotherapeutic potential was evaluated in a model of presensitized CD1 mice to peanut. After completing therapy, mice underwent an intraperitoneal challenge with peanut extract. Nanoparticle-treatment was associated with both less serious anaphylaxis symptoms and higher survival rates than control, confirming the protective effect of this formulation against the challenge. |
| Databáze: | OpenAIRE |
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