Hypoxia-ischemia brain damage disrupts brain cholesterol homeostasis in neonatal rats
| Autor: | Lv Sh, Yu Z, Piao H, Zhang J, Sun Ck, Ma H, Zhang Yh, Zhang Ym, Li S, Li Ap |
|---|---|
| Rok vydání: | 2010 |
| Předmět: |
medicine.medical_specialty
Central nervous system Enzyme-Linked Immunosorbent Assay Brain damage White matter Brain ischemia Rats Sprague-Dawley Microscopy Electron Transmission Internal medicine medicine Animals Analysis of Variance biology business.industry Interleukin-6 Tumor Necrosis Factor-alpha Age Factors Brain Myelin Basic Protein General Medicine medicine.disease Oligodendrocyte Myelin basic protein Rats medicine.anatomical_structure Endocrinology Cholesterol Animals Newborn Pediatrics Perinatology and Child Health Hypoxia-Ischemia Brain biology.protein Neuroglia Neurology (clinical) medicine.symptom business Immunostaining |
| Zdroj: | Neuropediatrics. 40(4) |
| ISSN: | 1439-1899 |
| Popis: | PURPOSE The first 3 weeks of life is the peak time of oligodendrocytes development and also the critical period of cholesterol increasing dramatically in central nervous system in rats. Neonatal hypoxia-ischemia (HI) brain damage happening in this period may disturb the brain cholesterol balance as well as white matter development. MATERIALS AND METHODS To test this hypothesis, postnatal day 7 (P7) Sprague-Dawley rats were subjected to HI insult. Cholesterol concentrations from brain and plasma were measured. White matter integrity was evaluated by densitometric analysis of myelin basic protein (MBP) immunostaining and electron microscopy. Brain TNF-alpha and IL-6 levels were also measured. RESULTS HI-induced brain cholesterol, but not the plasma cholesterol, levels decreased significantly during the first three days after HI compared with naive and sham operated rats (p |
| Databáze: | OpenAIRE |
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