Relationship between Productive HIV-1 Infection of Macrophages and CCR5 Utilization
| Autor: | Suzanne E. Pontow, Chia-Suei Hung, Lee Ratner |
|---|---|
| Rok vydání: | 1999 |
| Předmět: |
Receptors
CXCR4 Receptors CCR5 viruses Molecular Sequence Data HIV Envelope Protein gp120 Biology V3 loop Polymerase Chain Reaction CXCR4 Neutralization Flow cytometry Microbiology law.invention 03 medical and health sciences Neutralization Tests law Virology medicine Humans Macrophage Amino Acid Sequence Peptide sequence Cells Cultured Polymerase chain reaction Cell Line Transformed 030304 developmental biology Recombination Genetic 0303 health sciences medicine.diagnostic_test 030306 microbiology Macrophages Antibodies Monoclonal virus diseases Flow Cytometry Peptide Fragments 3. Good health CD4 Antigens DNA Viral HIV-1 Leukocytes Mononuclear biology.protein Antibody |
| Zdroj: | Virology. 264:278-288 |
| ISSN: | 0042-6822 |
| DOI: | 10.1006/viro.1999.0013 |
| Popis: | HIV-1 isolates exhibit specificity for infection of immortalized T-cell lines and macrophages. The distinct cellular tropisms have been attributed to expression of coreceptors CXCR4 or CCR5, respectively. However, it is unclear whether or not other tissue-specific determinants regulate entry. The current study uses a panel of viruses to analyze the relationship between CCR5 utilization and macrophage infection. Only chimeric viruses with the entire V3 loop from macrophage-tropic isolates, ADA or SF162, were able to infect macrophages. In contrast, chimeric viruses with smaller portions of the ADA V3 loop or the V3 loop of SF2, sufficient to allow CCR5 use, were insufficient for macrophage infection. PCR analysis showed that the defect in macrophage infection of the latter viruses was due to a defect in entry. Moreover, strains capable of infecting macrophages showed relative resistance to neutralization by anti-CCR5 antibody, 2D7, compared to strains which utilize CCR5 but are incapable of macrophage infection. |
| Databáze: | OpenAIRE |
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