Hexose uptake by Catharanthus roseus cell suspensions is inhibited by a high medium salt content
| Autor: | Marcel H. N. Hoefnagel, Kees R. Libbenga, L.H.W. van der Plas |
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| Rok vydání: | 1994 |
| Předmět: |
chemistry.chemical_classification
Chromatography Catharanthus roseus Apocynaceae Salt (chemistry) Fructose Plant Science General Medicine Carbohydrate Biology biology.organism_classification Hexose uptake Medium salts Salinity chemistry.chemical_compound chemistry Biochemistry Cell culture Hexose Laboratorium voor Plantenfysiologie EPS Agronomy and Crop Science Laboratory of Plant Physiology Cell suspensions |
| Zdroj: | Plant Cell Reports 13 (1994) Plant Cell Reports, 13, 464-467 |
| ISSN: | 1432-203X 0721-7714 |
| DOI: | 10.1007/bf00231968 |
| Popis: | The uptake of glucose and fructose from the medium by Catharanthus roseus cell suspensions was strongly inhibited by high medium salt concentration, such as found in LS (Linsmaier and Skoog 1965) medium. After inoculation into standard LS nutrient medium with less than 5 mM hexose no uptake occurred, while in low salt medium hexose was completely depleted. At a hexose concentration of 50 mM the uptake rate was higher in low salt medium than in standard medium. The lower rate of uptake at high salt concentration was not the result of a pH or osmotic effect of the salts. Probably the affinity of the hexose carrier is affected by the ion concentration of the medium. The decrease in medium salt concentration during normal batch culture probably will have a considerable effect on hexose uptake. |
| Databáze: | OpenAIRE |
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