Intranasal Administration of Antibody-Bound Respiratory Syncytial Virus Particles Efficiently Primes Virus-Specific Immune Responses in Mice
| Autor: | Ellen van der Schoot, Helga K. Einarsdottir, Debby Kruijsen, Frank E. J. Coenjaerts, Grada M. van Bleek, Gestur Vidarsson, Marcel A. Schijf |
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| Přispěvatelé: | Landsteiner Laboratory, Clinical Haematology |
| Rok vydání: | 2013 |
| Předmět: |
Palivizumab
medicine.drug_class viruses Immunology Antigen presentation Cellular Response to Infection Enzyme-Linked Immunosorbent Assay Receptors Fc Respiratory Syncytial Virus Infections Adaptive Immunity Biology Real-Time Polymerase Chain Reaction Monoclonal antibody Microbiology Virus Mice Neonatal Fc receptor Immune system Picrates Virology medicine Animals Administration Intranasal DNA Primers Mice Knockout Analysis of Variance Reverse Transcriptase Polymerase Chain Reaction Antigen processing Histocompatibility Antigens Class I Virion Flow Cytometry Antibodies Neutralizing Respiratory Syncytial Viruses Mice Inbred C57BL Insect Science biology.protein Cytokines Antibody medicine.drug |
| Zdroj: | Journal of virology, 87(13), 7550-7557. American Society for Microbiology |
| ISSN: | 1098-5514 0022-538X |
| Popis: | Infants are protected from a severe respiratory syncytial virus (RSV) infection in the first months of life by maternal antibodies or by prophylactically administered neutralizing antibodies. Efforts are under way to produce RSV-specific antibodies with increased neutralizing capacity compared to the currently licensed palivizumab. While clearly beneficial during primary infections, preexisting antibodies might affect the onset of adaptive immune responses and the ability to resist subsequent RSV infections. Therefore, we addressed the question of how virus neutralizing antibodies influence the priming of subsequent adaptive immune responses. To test a possible role of the neonatal Fc receptor (FcRn) in this process, we compared the responses in C57BL/6 wild-type (WT) and FcRn −/− mice. We observed substantial virus-specific T-cell priming and B-cell responses in mice primed with RSV IgG immune complexes resulting in predominantly Th1-type CD4 + T-cell and IgG2c antibody responses upon live-virus challenge. RSV-specific CD8 + T cells were primed as well. Activation of these adaptive immune responses was independent of FcRn. Thus, neutralizing antibodies that localize to the airways and prevent infection-related routes of antigen processing can still facilitate antigen presentation of neutralized virus particles and initiate adaptive immune responses against RSV. |
| Databáze: | OpenAIRE |
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